+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Coronavirus infection and demyelination. Sequence conservation of the S-gene during persistent infection of Lewis-rats

Coronavirus infection and demyelination. Sequence conservation of the S-gene during persistent infection of Lewis-rats

Advances in Experimental Medicine and Biology 440: 767-773

Coronaviruses display a large phenotypic variability, which may be an important factor for diversification and selection. Previous studies have demonstrated that the S-protein is an essential determinant of virulence and pathogenicity. Therefore we studied the S-gene as an indicator molecule for selection processes employing two different MHV-JHM variants. First, Lewis-rats were infected with MHV-JHM-Pi, a variant that causes demyelinating disease after several weeks p.i. It was not possible to isolate infectious MHV-JHM-Pi from such rats, although viral proteins were expressed. The S-gene was rescued directly from brain tissue employing RT-PCR technology. The amplicons were sequenced in bulk or at the level of single clones. We detected no evidence for an increase of S-gene mutants during the length of time. Only few mutations were found at the clonal level. The changes were distributed throughout the analysed S-gene fragments without a predilection in their location. The frequency of mutation remained low within a range of 0.03 to 0.5 mutations per thousand nucleotides. As a second approach, we sequenced the S-genes of viruses isolated from brain tissue infected with MHV-JHM-ts43. Infection of adult Lewis rats with that mutant resulted several weeks to months p.i. in demyelinating encephalomyelitis. The S-gene of this virus contains an insertion of 423 bp in the S1 region, which is identical to a polymorphic region described for MHV-4. In contrast to JHM-Pi, infectious MHV-JHM-ts43 was readily to isolate from brain tissue. The S-gene sequences of virus isolated 45-106 days p.i. from diseased rats were identical with that of the input virus. These results show, that during a persistent infection of Lewis-rats the S-gene was highly conserved.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 045652150

Download citation: RISBibTeXText

PMID: 9782356

Related references

Coronavirus infection and demyelination. Development of inflammatory lesions in Lewis rats. Advances in Experimental Medicine and Biology 440: 437-444, 1998

The Biology of Persistent Infection: Inflammation and Demyelination following Murine Coronavirus Infection of the Central Nervous System. Current Immunology Reviews 5(4): 267-276, 2009

No evidence for quasispecies populations during persistence of the coronavirus mouse hepatitis virus JHM: sequence conservation within the surface glycoprotein gene S in Lewis rats. Journal of General Virology 78: 747-756, 1997

Persistent Seoul virus infection in Lewis rats. Archives of Virology 149(7): 1325-1339, 2004

A leaderless genome identified during persistent bovine coronavirus infection is associated with attenuation of gene expression. Plos one 8(12): E82176, 2013

Autoimmune demyelination in primates following coronavirus infection. Neurology 42(4 Suppl. 3): 326, 1992

Expression of cellular oncogene Bcl-xL prevents coronavirus-induced cell death and converts acute infection to persistent infection in progenitor rat oligodendrocytes. Journal of Virology 79(1): 47-56, 2005

Demyelination induced by murine coronavirus JHM infection of congenitally immunodeficient mice. Advances in Experimental Medicine and Biology 380: 159-163, 1995

Effect of persistent and transient h pylori infection on h,k atpase and sialylated lewis antigens expression role of the host lewis gastric phenotype. Digestive Disease Week Abstracts & Itinerary Planner : Abstract No W885, 2003

Macrophage infiltration, but not apoptosis, is correlated with immune-mediated demyelination following murine infection with a neurotropic coronavirus. Journal of Virology 73(10): 8771-8780, 1999

CD26/DPP4 cell-surface expression in bat cells correlates with bat cell susceptibility to Middle East respiratory syndrome coronavirus (MERS-CoV) infection and evolution of persistent infection. Plos one 9(11): E112060, 2014

Reduced macrophage infiltration and demyelination in mice lacking the chemokine receptor CCR5 following infection with a neurotropic coronavirus. Virology 288(1): 8, 2001

A new model of persistent viral infection with primary demyelination. Neurologia Neurocirugia Psiquiatria 18(2-3 Suppl): 455-467, 1977

Dynamics of Lewis b binding and sequence variation of the babA adhesin gene during chronic Helicobacter pylori infection in humans. Mbio 5(6):, 2014

Persistent coronavirus infection of progenitor oligodendrocytes. Advances in Experimental Medicine and Biology 581: 379-384, 2006