+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Detection of feline coronavirus using RT-PCR: basis for the study of the pathogenesis of feline infectious peritonitis (FIP)



Detection of feline coronavirus using RT-PCR: basis for the study of the pathogenesis of feline infectious peritonitis (FIP)



Schweizer Archiv für Tierheilkunde 138(2): 74-79



The aim of this study was to further investigate the pathogenesis and epidemiology of feline coronavirus (FCoV)-infections and among others to determine the prognostic value of a positive result in the RT-PCR for FCoV in serum samples collected from cats with abdominal signs. Viral RNA was isolated from 100 microl of serum and subsequently amplified by a nested RT-PCR using primers binding to a highly conserved region of the 3'-end of the FCoV-genome. Sixty-three serum samples collected from 62 cats with abdominal signs were examined by RT-PCR and the clinical outcome was followed up. Four of these cats with a positive PCR-result are healthy more than 70 months after the collection of the blood sample. It can be concluded that viremia with FCoV does not necessarily lead to FIP and death. With respect to diagnosing FIP, a positive FCoV-RT-PCR is of low prognostic and diagnostic value. It can not be recommended to use this assay as sole indication to euthanize cats. Further studies will have to be carried out to demonstrate if the prognostic and diagnostic value of this PCR-assay in other samples such as peripheral blood mononuclear cells is more reliable. However, this method was found to be an important tool to further study the pathogenesis and epidemiology of FIP.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 045740413

Download citation: RISBibTeXText

PMID: 8720731


Related references

Detection of feline coronavirus RNA using RT-PCR: Basis for the study of the pathogenesis of feline infectious peritonitis (FIP). Schweizer Archiv fur Tierheilkunde 138(2): 74-79, 1996

Reverse Genetics for Type I Feline Coronavirus Field Isolate To Study the Molecular Pathogenesis of Feline Infectious Peritonitis. Mbio 9(4):, 2018

Perspectives on the epizootiology of feline enteric coronavirus and the pathogenesis of feline infectious peritonitis. Veterinary Microbiology 28(3): 243-256, 1991

Aspects of the pathogenesis and biology of feline infectious peritonitis virus and feline enteric coronavirus. Dissertation Abstracts International, B 44(1): 49-50, 1983

Detection of feline coronavirus antibody, feline immunodeficiency virus antibody, and feline leukemia virus antigen in ascites from cats with effusive feline infectious peritonitis. Journal of Veterinary Medical Science 66(1): 89-90, 2004

Acquisition of macrophage tropism during the pathogenesis of feline infectious peritonitis is determined by mutations in the feline coronavirus spike protein. Journal of Virology 79(22): 14122-14130, 2005

Detection of ascitic feline coronavirus RNA from cats with clinically suspected feline infectious peritonitis. Journal of Veterinary Medical Science 75(10): 1389-1392, 2013

Correlation of genomic detection of feline coronavirus with various diagnostic assays for feline infectious peritonitis. Journal of Veterinary Diagnostic Investigation 10(1): 93-97, 1998

Detection of feline coronavirus spike gene mutations as a tool to diagnose feline infectious peritonitis. Journal of Feline Medicine and Surgery 19(4): 321-335, 2017

Inflammatory Mediators in the Mesenteric Lymph Nodes, Site of a Possible Intermediate Phase in the Immune Response to Feline Coronavirus and the Pathogenesis of Feline Infectious Peritonitis?. Journal of Comparative Pathology 166: 69-86, 2019

Detection of feline coronavirus in cerebrospinal fluid for diagnosis of feline infectious peritonitis in cats with and without neurological signs. Journal of Feline Medicine and Surgery 18(2): 104-109, 2016

Detection of feline Coronavirus in effusions of cats with and without feline infectious peritonitis using loop-mediated isothermal amplification. Journal of Virological Methods 256: 32-36, 2018

Detection of feline Coronavirus in effusions of cats with and without feline infectious peritonitis using loop-mediated isothermal amplification. Journal of Virological Methods 256: 32-36, 2018

Detection of feline coronavirus mutations in paraffin-embedded tissues in cats with feline infectious peritonitis and controls. Journal of Feline Medicine and Surgery 21(2): 133-142, 2019

Comparative biological and biochemical properties of the feline coronaviruses feline infectious peritonitis virus and feline enteric coronavirus. Journal Of Cellular Biochemistry Supplementrt D: 297, 1986