Section 46
Chapter 45,746

Determination of Ro 48-3656 in rat plasma by reversed-phase high-performance liquid chromatography. Comparison of 1.5-microm nonporous silica to 3.5-microm porous silica analytical columns

Paasch, B.D.; Lin, Y.S.; Porter, S.; Modi, N.B.; Barder, T.J.

Journal of Chromatography. B Biomedical Sciences and Applications 704(1-2): 231-242


ISSN/ISBN: 1387-2273
PMID: 9518155
DOI: 10.1016/s0378-4347(97)00418-0
Accession: 045745574

We describe a method for measuring Ro 48-3656 in EDTA rat plasma by neutral pH, reversed-phase high-performance liquid chromatography using a 1.5-microm nonporous silica, C18 analytical column and UV absorbance detection to support pharmacokinetic studies. We also describe a comparison of the 1.5-microm nonporous silica C18 column versus 3.5-microm porous silica C18 columns. The final method using the 1.5-microm nonporous silica column demonstrated good precision (of both quantification and retention time), accuracy and recovery, linearity of dilution and limit of quantification (40 ng/ml Ro 48-3656 using a 20 microl injection). Samples of neat EDTA rat plasma were prepared by ultrafiltration followed by direct injection onto the HPLC column.

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