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Dissemination of MHV4 (strain JHM) infection does not require specific coronavirus receptors

Dissemination of MHV4 (strain JHM) infection does not require specific coronavirus receptors

Advances in Experimental Medicine and Biology 342: 279-284

In this report, we demonstrate the syncytial spread of MHV4 (strain JHM) infection through non-murine cell cultures which lack a specific MHV4 receptor and are therefore resistant to infection by free virions. This was achieved by allowing infected murine cells to settle onto confluent monolayers of non-murine cells in a straightforward infectious center assay. Receptor-independent syncytium formation induced by cells expressing the MHV4 spike (S) from recombinant vaccinia viruses (VV) indicated that spread was mediated by this coronavirus glycoprotein. We conclude that the S protein of MHV4 is so potently fusogenic that it does not require prior binding to a virus-specific surface receptor to induce fusion of closely-opposed plasma membranes.

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Accession: 045808327

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PMID: 8209743

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