+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Effect of glutamine and GABA on [U-(13)C]glutamate metabolism in cerebellar astrocytes and granule neurons



Effect of glutamine and GABA on [U-(13)C]glutamate metabolism in cerebellar astrocytes and granule neurons



Journal of Neuroscience Research 66(5): 885-890



To probe the effect of glutamine and GABA on metabolism of [U-(13)C]glutamate, cerebellar astrocytes were incubated with [U-(13)C]glutamate (0.5 mM) in the presence and absence of glutamine (2.5 mM) or GABA (0.2 mM). It could be shown that consumption of [U-(13)C]glutamate was decreased in the presence of glutamine and release of labeled aspartate and [1,2,3-(13)C]glutamate decreased as well, whereas the concentrations of these metabolites increased inside the cells. Glutamine decreased energy production from [U-(13)C]glutamate presumably by substituting for glutamate as an energy substrate. No additional effect was seen in the presence of both glutamine and GABA. When cerebellar granule neurons were incubated with [U-(13)C]glutamate (0.25 mM) and GABA (0.05 mM), less [U-(13)C]glutamate was used for energy production than in controls. Because the barbiturate thiopental did not elicit such response (Qu et al., 2000, Neurochem Int 37:207-215) it appears that GABA also has a metabolic function in the glutamatergic cerebellar granule neurons in contrast to the astrocytes.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 045879801

Download citation: RISBibTeXText

PMID: 11746415

DOI: 10.1002/jnr.10055


Related references

Effect of glutamate on glucose metabolism in cerebellar astrocytes and granule neurons by 13C MRS. Journal of Neurochemistry 78(Suppl. 1): 86, 2001

Compartmentation of glutamine, glutamate, and GABA metabolism in neurons and astrocytes: functional implications. Neuroscientist 9(5): 398-403, 2003

1-13C]glucose metabolism in brain cells: isotopomer analysis of glutamine from cerebellar astrocytes and glutamate from granule cells. Developmental Neuroscience 18(5-6): 460-468, 1996

[1- 13 C]Glucose Metabolism in Brain Cells: Isotopomer Analysis of Glutamine from Cerebellar Astrocytes and Glutamate from Granule Cells. Developmental Neuroscience 18(5-6): 460-468, 1996

Arachidonic acid inhibits uptake of glutamate and glutamine but not of GABA in cultured cerebellar granule cells. Journal of Neuroscience Research 17(4): 424-427, 1987

Differences between neurons and astrocytes in glutamine and glutamate metabolism. Journal of Neurochemistry 65(Suppl. ): S125, 1995

Metabolism and release of glutamate in cerebellar granule cells cocultured with astrocytes from cerebellum or cerebral cortex. Journal of Neurochemistry 56(1): 59-66, 1991

The glutamine-glutamate/GABA cycle: function, regional differences in glutamate and GABA production and effects of interference with GABA metabolism. Neurochemical Research 40(2): 402-409, 2015

U-13C] aspartate metabolism in cultured cortical astrocytes and cerebellar granule neurons studied by NMR spectroscopy. Glia 23(3): 271-277, 1998

Glutamate is preferred over glutamine for intermediary metabolism in cultured cerebellar neurons. Journal of Cerebral Blood Flow and Metabolism 27(4): 811-820, 2006

Effects of thiopental on transport and metabolism of glutamate in cultured cerebellar granule neurons. Neurochemistry International 37(2-3): 207-215, Aug -Sept, 2000

Effects of pentylenetetrazole and glutamate on metabolism of [U-(13)C]glucose in cultured cerebellar granule neurons. Neurochemistry International 40(2): 181-187, 2002

Antagonists of the nmda sensitive glutamate receptor down regulate messenger rna encoding various gaba receptor subunits in cerebellar granule neurons. Society for Neuroscience Abstracts 16(1): 75, 1990

Stimulation of the NMDA selective glutamate receptor changes GABA-A receptor subunit proteins and mRNAs in primary cultures of rat cerebellar granule neurons. Society for Neuroscience Abstracts 19(1-3): 475, 1993

Inhibitors of the alpha-ketoglutarate dehydrogenase complex alter [1-13C]glucose and [U-13C]glutamate metabolism in cerebellar granule neurons. Journal of Neuroscience Research 83(3): 450-458, 2006