Effect of oral administration of vitamin C on human aqueous humor ascorbate concentration
Iqbal, Z.; Midgley, J.M.; Watson, D.G.; Karditsas, S.D.; Dutton, G.N.; Wilson, W.S.
Zhongguo Yao Li Xue Bao 20(10): 879-883
ISSN/ISBN: 0253-9756 PMID: 11270984 Accession: 045886338
To study oral administration of vitamin C on human aqueous humour ascorbate concentration. High performance liquid chromatography (HPLC) coupled with electrochemical detector (ECD) was used. The effect of oral administration of various doses of ascorbic acid, 0 (control), 1.0, 1.5, 2.0, 3.0, and 5.0 g, on its concentration in aqueous humour, obtained from volunteer cataract patients was studied. The concentration of ascorbic acid in aqueous humour of control group (without administration of vitamin-C tablet or drug containing ascorbic acid was (254 +/- 119) mg.L-1. This study revealed that the administration of 2.0 g of ascorbic acid saturate the aqueous humour and further increase in the dose (3.0 g and 5.0 g) did not increase its concentration in aqueous humour, although its concentration was increased in plasma. Oral administration of 2.0 g of Vc is sufficient to saturate the aqueous humour where it may be helpful in controlling the intra-ocular pressure.