Effective binding of alpha-bungarotoxin with the solubilized alpha-subunit of Torpedo californica acetylcholine receptor
Utkin, I.N.; Mund, M.; Hucho, F.; Tsetlin, V.I.
Bioorganicheskaia Khimiia 22(5): 387-389
1996
ISSN/ISBN: 0132-3423 PMID: 8929226 Accession: 045896105
alpha-Subunit of the Torpedo californica nicotinic acetylcholine receptor was isolated by preparative SDS-PAGE followed by reversed-phase HPLC on a C4 column in an acetonitrile-isopropanol gradient in water. After removal of the organic solvents and solubilization in beta-octylglucoside, the purified alpha-subunit binds alpha-bungarotoxin with high affinity (Kd 28 nM).
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