+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Effects of ozone on ovalbumin sensitization in guinea pigs

Effects of ozone on ovalbumin sensitization in guinea pigs

Journal of Microbiology, Immunology, and Infection 31(4): 225-232

The effects of ozone on allergen sensitization is still controversial. This study is aimed to clarify the effect of ozone on airway hypersensitivity to ovalbumin (OVA) in guinea pigs, by exposing them to ozone (3 ppm 2 hours daily) and OVA (1 gm/dL 10 min weekly) for two weeks. OVA specific IgG and airway hypersensitivity to methacholine were determined after the sensitization. The results demonstrated that there was a synergistic effect of ozone on OVA-induced IgG antibody production. OVA specific IgG was 0.51 +/- 0.1 U/mL and 0.46 +/- 0.1 U/mL using OVA and ozone alone respectively, which increased to 1.09 +/- 0.4 U/mL when both OVA and ozone were given simultaneously. Ozone exposure was able to cause airway hypersensitivity. The provocation dose of methacholine causing a 100% increase of airway resistance (PD 100 airway resistance) was 0.54 +/- 0.19 mg/mL and 1.17 +/- 0.30 mg/mL using OVA and ozone exposure alone, which had decreased to 0.49 +/- 0.18 mg/mL when both OVA and ozone were given simultaneously. There were macrophages around the peribronchial tissue in all guinea pigs except in the control. The macrophages in the group of OVA with ozone (24.2 +/- 7.2 cells/HPF) were significantly more than those in the OVA group (18.0 +/- 4.2 cells/HPF) and the ozone group (14.5 +/- 6.2 cells/HPF). When OVA-presensitized guinea pigs were exposed to ozone immediately or daily for two weeks, a restrictive type of lung function impairment with similar airway hypersensitivity post ozone exposure was observed. In conclusion, guinea pigs exposed to ozone in conjunction with aeroallergen may not only facilitate the damage of the airway but also enhance antibody production to the aeroallergen. The ozone-induced airway inflammation might have a contributing effect on OVA induced airway hypersensitivity in guinea pigs.

(PDF emailed within 1 workday: $29.90)

Accession: 045917658

Download citation: RISBibTeXText

PMID: 10496163

Related references

Effects of estradiol on the course of ovalbumin sensitization in guinea pigs. Acta Pharmacologica Sinica 14(1): 49-51, 1993

Effects of ozone exposure on experimental asthma in guinea pigs sensitized with ovalbumin through the airway. International Archives of Allergy and Applied Immunology 93(2-3): 139-147, 1990

Effects of sensitization to ovalbumin on the lipid content of the bronchoalveolar lavage fluid in guinea pigs. Bulletin Europeen de Physiopathologie Respiratoire 21(4): 331-338, 1985

Bronchial sensitization in guinea pigs following ingestion of ovalbumin. International Archives of Allergy and Applied Immunology 90(4): 395-399, 1989

Effect of a combination of 3 pesticides on the sensitization of guinea pigs to ovalbumin. Eksperimentalna Meditsina i Morfologiia 18(1): 22-24, 1979

Bronchoconstriction by inhaled adenosine in guinea pigs pretreated with ovalbumin paf or ozone. British Journal of Pharmacology 104(PROC SUPPL DEC): ABSTRACT 400P, 1991

Effect of ozone on response to ovalbumin & its modulation by vitamins C & E in sensitized guinea pigs. Indian Journal of Medical Research 132: 87-93, 2010

Ovalbumin sensitization alters the ventilatory responses to chemical challenges in guinea pigs. Journal of Applied Physiology 99(5): 1782-1788, 2005

Effect of respiratory syncytial virus on subsequent allergic sensitization to ovalbumin in guinea-pigs. European Respiratory Journal 13(5): 976-982, 1999

Passive sensitization of human skin by sera of guinea pigs anaphylactically sensitized to ovalbumin. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 77(2): 245-247, 1951

Secretory phospholipase A-2 activity is elevated bronchoalveolar lavage fluid after ovalbumin sensitization of guinea pigs. Journal of Leukocyte Biology 60(6): 704-709, 1996

Ovalbumin sensitization of guinea-pigs reduces fMLP-induced calcium signal in alveolar macrophages. Life Sciences 69(14): 1597-1607, August 24, 2001