+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Enzymatic amplification and expression of bovine interleukin-1 receptor antagonist cDNA



Enzymatic amplification and expression of bovine interleukin-1 receptor antagonist cDNA



Veterinary Immunology and Immunopathology 62(3): 197-208



cDNA generated from lipopolysaccharide-stimulated bovine peripheral blood mononuclear cells was used to amplify and clone the bovine interleukin-1 receptor antagonist (IL-1ra) using primers derived from semi-conserved regions between human and mouse IL-1ra sequences. 5' and 3' terminal sequences of bovine IL-1ra were amplified by 5' and 3' rapid amplification of cDNA ends. The deduced amino acid sequence of bovine IL-1ra demonstrated 80%, 78%, 78%, 77% and 76% homology with human, mouse, rat, rabbit and equine sequences, respectively. Recombinant bovine IL-1ra produced in Escherichia coli suppressed the growth inhibitory activity of bovine IL-1beta on A375 cells in a dose-dependent manner, indicating that the present bovine IL-1ra cDNA encodes biologically active proteins.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 045973385

Download citation: RISBibTeXText

PMID: 9643454

DOI: 10.1016/s0165-2427(98)00099-3


Related references

Enzymatic amplification and molecular cloning of cDNA encoding the small and large subunits of bovine interleukin 12. Biochimica Et Biophysica Acta 1270(2-3): 215-217, 1995

Determination of the cDNA sequence and mRNA expression of interleukin-1 receptor antagonist in horses. American Journal of Veterinary Research 61(8): 920-924, 2000

CDNA cloning and gene expression of the type 1 bovine interleukin-1 receptor. Veterinary Immunology and Immunopathology 71(3/4): 245-255, 1999

Allele 2 of the interleukin-1 receptor antagonist gene polymorphism is associated with reduced expression of interleukin-1 receptor antagonist in ulcerative colitis. Gut 42(Suppl. 1): A48, 1998

Inhibition of interleukin-1 (alpha and beta), interleukin-2 secretion and surface expression of interleukin-2 receptor (IL-2R) by a novel cytokine interleukin-1 receptor antagonist (IL-1ra). Scandinavian Journal of Immunology 36(1): 27-33, 1992

Inhibition of Interleukin-1 (Alpha and Beta), Interleukin-2 Secretion and Surface Expression of Interleukin-2 Receptor (IL-2R) by a Novel Cytokine Interleukin-1 Receptor Antagonist (IL-lra). Scandinavian Journal of Immunology 36(1): 27-34, 1992

Expression of interleukin-1beta, interleukin-1 receptor, and interleukin-1 receptor antagonist mRNA in rat carotid artery after balloon angioplasty. Biochemical and Biophysical Research Communications 271(1): 138-143, 2000

Coordinated antiinflammatory effects of interleukin 4: interleukin 4 suppresses interleukin 1 production but up-regulates gene expression and synthesis of interleukin 1 receptor antagonist. Proceedings of the National Academy of Sciences of the United States of America 89(9): 4076-4080, 1992

Interleukin 1-beta, interleukin 1 receptor antagonist, interleukin 10, and interleukin 13 gene expression in the central nervous system and anterior pituitary during systemic inflammation Pathophysiological implications. Journal of Investigative Medicine 45(3): 238A, 1997

Polymorphism in intron 2 of the fetal interleukin-1 receptor antagonist genotype influences midtrimester amniotic fluid concentrations of interleukin-1beta and interleukin-1 receptor antagonist and pregnancy outcome. American Journal of Obstetrics and Gynecology 189(5): 1413-1417, 2003

Different pattern of interleukin-1 beta-(IL-1 beta), interleukin-1 receptor antagonist- (IL-1ra) and interleukin-1 receptor type I- (IL-1R tI) mRNA-expression in single preimplantation mouse embryos at various developmental stages. Journal of Reproductive Immunology 34(2): 103-120, 1997

Characterization of a cDNA encoding bovine interleukin 10: Kinetics of expression in bovine lymphocytes. Gene (Amsterdam) 139(2): 257-261, 1994

Description of an Atlantic salmon (Salmo salar L.) type II interleukin-1 receptor cDNA and analysis of interleukin-1 receptor expression in amoebic gill disease-affected fish. Fish and Shellfish Immunology 32(6): 1185-1190, 2012

Expression of interleukin-1 alpha, interleukin-1 beta, and an interleukin-1 receptor antagonist in human retinal pigment epithelial cells. Experimental Eye Research 55(2): 325-335, 1992

Inhibition of kainic acid induced expression of interleukin-1 beta and interleukin-1 receptor antagonist mRNA in the rat brain by NMDA receptor antagonists. Brain Research. Molecular Brain Research 85(1-2): 103-113, 2000