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Enzymatic amplification and expression of bovine interleukin-1 receptor antagonist cDNA

Enzymatic amplification and expression of bovine interleukin-1 receptor antagonist cDNA

Veterinary Immunology and Immunopathology 62(3): 197-208

cDNA generated from lipopolysaccharide-stimulated bovine peripheral blood mononuclear cells was used to amplify and clone the bovine interleukin-1 receptor antagonist (IL-1ra) using primers derived from semi-conserved regions between human and mouse IL-1ra sequences. 5' and 3' terminal sequences of bovine IL-1ra were amplified by 5' and 3' rapid amplification of cDNA ends. The deduced amino acid sequence of bovine IL-1ra demonstrated 80%, 78%, 78%, 77% and 76% homology with human, mouse, rat, rabbit and equine sequences, respectively. Recombinant bovine IL-1ra produced in Escherichia coli suppressed the growth inhibitory activity of bovine IL-1beta on A375 cells in a dose-dependent manner, indicating that the present bovine IL-1ra cDNA encodes biologically active proteins.

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Accession: 045973385

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PMID: 9643454

DOI: 10.1016/s0165-2427(98)00099-3

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