+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Evaluation of a tissue-engineered membrane-cell construct for guided bone regeneration



Evaluation of a tissue-engineered membrane-cell construct for guided bone regeneration



International Journal of Oral and Maxillofacial Implants 17(2): 161-174



Currently, a number of bioresorbable and biodegradable membranes used for guided bone regeneration lead to incomplete tissue regeneration. Poor mechanical properties, short degradation time, and the lack of integrated biologic components result in the inability to create and maintain an appropriate environment and to actively support tissue remodeling. In the present study, the osteogenic potential of human calvarial periosteal cells in combination with ultrathin polycaprolactone (pc2) membranes of a slow biodegradation rate was investigated. In vitro and in vivo analyses of the tissue-engineered constructs were conducted using imaging techniques, immunohistochemistry, and histology. Two types of membranes were investigated. Group 1 consisted of a plain membrane, and in group 2 membranes were treated with sodium hydroxide. In vitro results showed that osteoblast-like cells attached and proliferated on the membranes with the formation of extracellular matrix. Sodium hydroxide-treated membranes showed enhanced cell attachment and proliferation kinetics, resulting in a dense cellular layer after 2 weeks in culture. In vivo mineralized tissue formation in association with vascularization was observed. Extracellular matrix calcification with nodule formation was detected via histology as well as scanning electron microscopy. PCL membranes support the attachment, growth, and osteogenic differentiation of human primary osteoblast-like cells. Sodium hydroxide-treated membranes demonstrated increased cell attachment resulting from increased hydrophilicity. These findings have potential application in the development of a new generation of osteoconductive membranes.

(PDF emailed within 1 workday: $29.90)

Accession: 046007037

Download citation: RISBibTeXText

PMID: 11958398


Related references

Evaluation of guided tissue regeneration in interproximal defects. (II). Membrane and bone versus membrane alone. Journal of Clinical Periodontology 23(5): 485-491, 1996

Evaluation of a poly-l-lactic acid membrane and membrane fixing pin for guided tissue regeneration on bone defects in dogs. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics 97(2): 155-163, 2004

A Cell-Engineered Small Intestinal Submucosa-Based Bone Mimetic Construct for Bone Regeneration. Tissue Engineering. Part a 24(13-14): 1099-1111, 2018

Guided tissue regeneration employing a collagen membrane in a human periodontal bone defect: a histologic evaluation. International Journal of Periodontics and Restorative Dentistry 17(3): 282-291, 1997

A clinical evaluation of guided tissue regeneration with a bioabsorbable matrix membrane combined with an allograft bone graft. A series of case reports. Journal of Periodontology 68(6): 598-607, 1997

Evaluation of a cell-permeable barrier for guided tissue regeneration combined with demineralized bone matrix. Clinical Oral Implants Research 14(6): 812-818, 2004

Evaluation of bone formation and membrane degradation in guided bone regeneration using a 4-hexylresorcinol-incorporated silk fabric membrane. Maxillofacial Plastic and Reconstructive Surgery 37(1): 32, 2015

Mechanical evaluation of a tissue-engineered zone of calcification in a bone-hydrogel osteochondral construct. Computer Methods in Biomechanics and Biomedical Engineering 18(3): 332-337, 2015

Intervertebral disc regeneration with an adipose mesenchymal stem cell-derived tissue-engineered construct in a rat nucleotomy model. Acta Biomaterialia 2019, 2019

Guided bone regeneration using a bone tissue engineering complex consisting of a poly- dl -lactide membrane and bone mesenchymal stem cells. Oncotarget 9(23): 16380-16388, 2018

Adipose stem cell tissue-engineered construct used to treat large anterior mandibular defect: a case report and review of the clinical application of good manufacturing practice-level adipose stem cells for bone regeneration. Journal of Oral and Maxillofacial Surgery 71(5): 938-950, 2013

A comparative evaluation of freeze-dried bone allograft with and without bioabsorbable guided tissue regeneration membrane Healiguide(®) in the treatment of Grade II furcation defects: A clinical study. Journal of Indian Society of Periodontology 19(6): 645-650, 2016

Evaluation of the biocompatibility and cell segregation performance of acellular dermal matrix as barrier membrane on guided tissue regeneration in vitro. Shanghai Kou Qiang Yi Xue 22(3): 260-264, 2015

Comparison of effect between vascularization osteogenesis and membrane guided osteogenesis in bone repair by tissue engineered bone with pedicled fascial flap packing autologous red bone marrow. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 25(6): 729-735, 2012

A comparative evaluation of the effectiveness of guided tissue regeneration by using a collagen membrane with or without decalcified freeze-dried bone allograft in the treatment of infrabony defects: A clinical and radiographic study. Journal of Indian Society of Periodontology 17(4): 484-489, 2013