+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Evaluation of developing rat testis by phosporus 31P magnetic resonance spectroscopy and DNA flowcytometry



Evaluation of developing rat testis by phosporus 31P magnetic resonance spectroscopy and DNA flowcytometry



Pediatric Surgery International 17(4): 316-320



For the assessment of germ-cell maturation of the seminiferous tubules, DNA flowcytometry is a rapid and sensitive method. Phosporus 31P magnetic resonance spectroscopy (MRS) is a non-invasive alternative that demonstrates the metabolic status of the testis, reflecting the type and relative proportion of germ cells in the testis. This study was designed to evaluate the utility of 31P MRS in reflecting the haploid-cell population of the testis as measured by DNA flowcytometry. A single testicle was evaluated in male Wistar pre-pubertal rats at 30, 40, 50, and 60 days of age. In order to minimize the contamination of signals from the contralateral testis, scrotum, and tail, the technique was modified and the testis was evaluated ex-vivo with an intact blood supply. At 30 days of age the percentage of haploid cells was 43.6 +/- 1.8, and this increased to 72.7 +/- 1.4 at 60 days of age. During this period, the testicular phosphomonoester/adenosine triphosphate (PM/ATP) ratio changed from 1.70 +/- 0.21 to 0.32 +/- 0.08. There was a significant (P < 0.001) linear correlation between the proportion of haploid cells evaluated by DNA flowcytometry and the PM/ATP ratio evaluated by 31P MRS. 31P MRS is thus a reliable, noninvasive technique for accurately assessing the status of the seminiferous epithelium.

(PDF emailed within 0-6 h: $19.90)

Accession: 046009112

Download citation: RISBibTeXText

PMID: 11409170

DOI: 10.1007/s003830100602


Related references

In vivo 1H nuclear magnetic resonance spectroscopy with a 94T magnet for evaluation of hippocampal metabolism in the developing rat brain. Pediatric Research 45(4 PART 2): 346A, 1999

In Vivo 1H Nuclear Magnetic Resonance Spectroscopy (MRS) with a 9.4T Magnet for Evaluation of Hippocampal Metabolism in the Developing Rat Brain. Pediatric Research 45(4, Part 2 of 2): 346a-346a, 1999

Evaluation of potential effectors of agonal glycolytic rate in developing brain measured in vivo by 31P and 1H nuclear magnetic resonance spectroscopy. Journal Of Neurochemistry. 64(1): 322-331, 1995

Functional and metabolic evaluation of the athlete's heart by magnetic resonance imaging and dobutamine stress magnetic resonance spectroscopy. Circulation 97(7): 666-672, 1998

Diffusion-weighted magnetic resonance imaging and magnetic resonance spectroscopy in the evaluation of focal cerebral tubercular lesions. Acta Radiologica 45(6): 679-688, 2004

Evaluation of neurodegeneration in a mouse model of infantile batten disease by magnetic resonance imaging and magnetic resonance spectroscopy. Neuro-Degenerative Diseases 9(4): 159-169, 2012

Evaluation of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes with magnetic resonance imaging and proton magnetic resonance spectroscopy. Chinese Medical Sciences Journal 21(4): 234-238, 2007

Evaluation of transient apical ballooning with cardiac magnetic resonance imaging and 31-phosphorous magnetic resonance spectroscopy. International Journal of Cardiology 118(2): 249-252, 2006

Magnetic resonance imaging and proton magnetic resonance spectroscopy of the brain in the diagnostic evaluation of developmental delay. Yearbook of Diagnostic Radiology 2010: 150-151, 2010

Magnetic resonance imaging/magnetic resonance spectroscopy biomarkers evaluation of stunned myocardium in canine model. Investigative Radiology 46(4): 209-214, 2011

Magnetic resonance imaging and proton magnetic resonance spectroscopy of the brain in the diagnostic evaluation of developmental delay. European Journal of Paediatric Neurology 13(2): 181-190, 2008

Role of Magnetic Resonance Imaging, Magnetic Resonance Spectroscopy and Transrectal Ultrasound in Evaluation of Prostatic Pathologies with Focus on Prostate Cancer. Polish Journal of Radiology 82: 827-836, 2018

MRI/MRS evaluation of cariporide in a canine long-term model of reperfused ischemic insults. Magnetic resonance imaging/magnetic resonance spectroscopy. Journal of Magnetic Resonance Imaging 17(1): 136-141, 2002

The evaluation of human breast lesions with magnetic resonance imaging and proton magnetic resonance spectroscopy. Breast Cancer Research & Treatment 68(1): 45-54, 2001

Quantitative evaluation of improvement in muscle weakness in a patient receiving extracorporeal photopheresis for scleroderma: magnetic resonance imaging and magnetic resonance spectroscopy. Journal of the American Academy of Dermatology 33(3): 519-522, 1995