+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Evaluation of different sperm function tests as screening methods for male fertilization potential--the value of the sperm migration test



Evaluation of different sperm function tests as screening methods for male fertilization potential--the value of the sperm migration test



Fertility and Sterility 62(3): 591-598



To assess the value of different sperm function screening tests in predicting fertilization. Prospective study. Academic tertiary referral center for fertility treatment. Ninety-five couples attending for initial screening and IVF-ET. Only cycles where three or more grade I oocytes were collected were included, and patients with endometriosis were excluded. Each patient had a standard semen analysis, cervical mucus (CM) penetration test, hypo-osmotic swelling test, and sperm migration test performed between 4 and 8 weeks before IVF-ET. The correlation between sperm function test results and the percentage of fertilized oocytes and the power of the tests to predict fertilization. The sperm migration test correlated highly with fertilization rate (r = 0.62) and was most useful in identifying the group of patients likely to achieve fertilization (Odds ratio [OR] 0.07, confidence interval [CI] 0.02 to 0.2). The CM penetration test showed a moderate correlation with fertilization rate (r = 0.45) and some predictive power (OR 0.37, CI 0.13 to 1.00). Sperm concentration, but not motility or normal morphology, showed slight correlation with fertilization rate (r = 0.28) but the combination of normal semen parameters did not distinguish patients likely to achieve fertilization (OR 1.51, CI 0.62 to 3.65). The hypo-osmotic swelling test did not correlate with fertilization rate (r = 0.21). This study evaluated the predictive power of several simple tests available for use in most laboratories as screening tests of sperm fertilization potential. Apart from sperm concentration, normal traditional semen characteristics were of little clinical benefit. The hypo-osmotic swelling test had no predictive power. The CM penetration test correlated with fertilization rate but might be difficult to perform routinely as a continuous supply of suitable CM would be required. The sperm migration test proved to be the best discriminator of sperm fertilization potential and should be considered as a first level screening test in the assessment of male fertility.

(PDF emailed within 0-6 h: $19.90)

Accession: 046009232

Download citation: RISBibTeXText

PMID: 8062956

DOI: 10.1016/s0015-0282(16)56951-9


Related references

A new test for the assessment of sperm-zona pellucida penetration: relationship with results of other sperm tests and fertilization in vitro. Human Reproduction 9(3): 489-496, 1994

Male partner screening before in vitro fertilization: Preselecting patients who require intracytoplasmic sperm injection with the sperm penetration assay. Fertility & Sterility 76(6): 1113-1118, 2001

Effects of microsurgical varicocelectomy on semen analysis and sperm function tests in patients with different grades of varicocele: Role of sperm functional tests in evaluation of treatments outcome. Andrologia 50(8): E13069, 2018

Evaluation of Sperm 24-Hour Motility and Sperm Recovery After Density Gradient Separation in Relation to Fertilization Potential. Fertility and Sterility 84: S459-S460, 2005

Value of the sperm deoxyribonucleic acid fragmentation level, as measured by the sperm chromatin dispersion test, in the outcome of in vitro fertilization and intracytoplasmic sperm injection. Fertility and Sterility 85(2): 371-383, 2006

The single sperm curling test, a modified hypo-osmotic swelling test, as a potential technique for the selection of viable sperm for intracytoplasmic sperm injection. Fertility & Sterility 68(2): 346-350, 1997

An evaluation of the efficacy of in vitro fertilization with intracytoplasmic sperm injection for sperm with low hypoosmotic swelling test scores and poor morphology. Journal of Assisted Reproduction & Genetics 20(5): 182-185, 2003

Prognostic value of two putative sperm function tests: hypo-osmotic swelling and bovine sperm mucus penetration test (Penetrak). Human Reproduction 7(9): 1240-1244, 1992

Failed fertilization following intracytoplasmic sperm injection with testicular sperm Evaluation of sperm chromatin decondensation. Journal of Urology 157(4 SUPPL ): 446, 1997

Inhibition of human sperm function and mouse fertilization in vitro by an antibody against cation channel of sperm 1: the contraceptive potential of its transmembrane domains and pore region. Fertility and Sterility 92(3): 1141-1146, 2008

An evaluation of the mouse sperm morphology test and other sperm tests in nonhuman mammals. A report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutation Research 115(1): 1-72, 1983

Tests of sperm function for the evaluation of the male penetrak and tru trax. Journal of Urology 147(4 SUPPL): 323A, 1992

Tests of sperm function for evaluation of the male: Penetrak and Tru-Trax. Fertility and Sterility 60(2): 319-323, 1993

Evaluation of sperm preparation methods in in vitro fertilization and embryo transfer for male infertility. Japanese Journal of Fertility & Sterility 38(4): 618-623, 1993

Prediction of the in-vitro fertilization (IVF) potential of human spermatozoa using sperm function tests: the effect of the delay between testing and IVF. Human Reproduction 11(5): 1030-1034, 1996