+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Evaluation of four rapid methods for the investigation of the toxigenic capacity of Clostridium difficile strains isolated in a selective medium

Evaluation of four rapid methods for the investigation of the toxigenic capacity of Clostridium difficile strains isolated in a selective medium

Enfermedades Infecciosas Y Microbiologia Clinica 18(3): 109-112

Use of selective Clostridium difficile culture as a diagnostic method for C. difficile associated disease requires to prove the toxigenic ability of the isolates. Toxin B detection by cell culture assay after growing the microorganism in enriched broth is the standard method, but it delays the final diagnosis for 3-5 days. This study compares retrospectively four rapid techniques for detecting these toxigenic C. difficile strains. 106 clinical isolates of C. difficile (72 toxigenic and 34 non-toxigenic), these and 16 clinical strains of other species of Clostridium were investigated. The four methods were performed directly from colonies growing on solid agar. They were: a) cytotoxin detection in cell culture; b) two PCR amplifications of toxin A and toxin B, respectively, and c) toxin A detection by an immunoenzymatic method (VIDAS CDA2). All these procedures were completed within a normal working day. Only the 72 toxigenic C. difficile strains gave positive results by cell culture and PCR techniques (sensitivity and specificity: 100%). A total of 14 out of 49 toxigenic C. difficile strains showed negative results by the VIDAS assay in the first run, but all them were positive in repeated tests. Although all methods performed well, the cytotoxicity assay done directly on colonies growing in CCFA is a simple and rapid technique, and appears to be well-suited for use in laboratories with access to cell cultures.

(PDF emailed within 1 workday: $29.90)

Accession: 046009946

Download citation: RISBibTeXText

PMID: 10905010

Related references

Multicenter clinical evaluation of the portrait toxigenic C. difficile assay for detection of toxigenic Clostridium difficile strains in clinical stool specimens. Journal of Clinical Microbiology 50(12): 3932-3936, 2013

Evaluation of a new automated homogeneous PCR assay, GenomEra C. difficile, for rapid detection of Toxigenic Clostridium difficile in fecal specimens. Journal of Clinical Microbiology 51(9): 2908-2912, 2014

Rapid detection of toxigenic Clostridium difficile strains by a nested PCR of the toxin B gene. Clinical Microbiology and Infection 3(1): 145-147, 1997

Rapid detection of toxigenic strains of Clostridium difficile in diarrheal stools by real-time PCR. Journal of Clinical Microbiology 47(4): 1276-1277, 2009

Identification of toxigenic Clostridium difficile strains isolated from alimentary tract of dogs by PCR. Medycyna Doswiadczalna i Mikrobiologia 46(3): 201-206, 1994

Clostridium difficile infection diagnostics - evaluation of the C. DIFF Quik Chek Complete assay, a rapid enzyme immunoassay for detection of toxigenic C. difficile in clinical stool samples. Apmis 124(11): 1016-1020, 2016

Evaluation of a novel molecular assay to diagnose toxigenic strains of Clostridium difficile. Anaerobe 52: 111-114, 2018

Evaluation of rapid enzyme immunoassays for clostridium difficile toxin a in comparison to standard methods for the diagnosis of clostridium difficile associated disease. Program & Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 32: 319, 1992

Haemagglutination activity of toxigenic and non-toxigenic strains of Clostridium difficile. Fems Microbiology Letters. 118(1-2): 141-144, 1994

Evaluation of Xpert C. difficile, BD MAX Cdiff, IMDx C. difficile for Abbott m2000, and Illumigene C. difficile Assays for Direct Detection of Toxigenic Clostridium difficile in Stool Specimens. Annals of Laboratory Medicine 36(2): 131-137, 2017

A cost-effective approach for detection of toxigenic Clostridium difficile: toxigenic culture using ChromID Clostridium difficile agar. Journal of Clinical Microbiology 52(2): 671-673, 2014

The actual condition of laboratory diagnostic of Clostridium difficile-associated diarrhea; the methods of detection of toxigenic strains (review of publications). Klinicheskaia Laboratornaia Diagnostika 62(10): 635-640, 2019

Nontoxigenic Clostridium difficile protects hamsters against challenge with historic and epidemic strains of toxigenic BI/NAP1/027 C. difficile. Antimicrobial Agents and ChemoTherapy 57(11): 5266-5270, 2014

Testing characteristics of VIDAS Clostridium difficile Toxin A II, Premier Toxins AB, cytotoxin assay and bacterial culture for the detection of toxigenic strains of C difficile. Abstracts of the General Meeting of the American Society for Microbiology 103: C-015, 2003

Evaluation of four methods for detection of Clostridium difficile or C. difficile toxin: cytotoxin assay, culture, latex agglutination, and a new rapid immunoassay (C. difficile toxin A test). Zeitschrift für Gastroenterologie 36(2): 143-149, 1998