+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Expansion of memory Th2 cells over Th1 cells in neonatal primed mice



Expansion of memory Th2 cells over Th1 cells in neonatal primed mice



Transplantation 60(11): 1187-1193



BALB/c mice primed with CAF1 splenocytes during the neonatal stage developed A/J-specific tolerance with prolonged survival (> 60 days) of A/J skin grafts. Mice failed to develop A/J-specific cytotoxicity, but rejected third-party skin grafts and generated appropriate third-party cytotoxic T cell responses. We demonstrated previously that graft acceptance was associated with enhanced interleukin (IL)-4 and diminished interferon [IFN]-gamma tolerogen-specific cytokine production, whereas third-party graft rejection was associated with the opposite pattern of cytokine production. We now report that neonatal mice do not mount mixed lymphocyte reaction responses against A/J, but the mice contain a higher percentage of IL-4-producing cells that were characterized as CD4+Mel-14lo cells. Although alloantigen priming of both neonatal and adult control mice expands the CD4+Mel-14lo subset, CD4+Mel-14lo cells from neonatal primed mice produce significantly higher levels of IL-4 and IL-10 and lower IFN-gamma, whereas CD4+Mel-14lo cells from adult primed mice produce mainly IFN-gamma. Moreover, enzyme-linked spot immunosorption analysis demonstrates that, compared with adult primed mice, neonatal primed mice contain more IL-4-producing CD4 cells and less IFN-gamma-producing cells, which indicates that neonatal antigen exposure induces and expands alloreactive Th2 memory CD4 cells. The addition of neutralizing antibodies against IL-4 and IL-10 to primary MLR failed to recover IFN-gamma by CD4+Mel-14lo cells, but cells secreted IFN-gamma after a second in vitro restimulation with tolerogen, which indicates that CD4 cells from neonatal tolerant mice have the capacity to differentiate into Th1 cells. In summary, neonatal tolerant mice contain higher ratios of Th2/Th1 CD4 cells, and the Th2 cytokines function to maintain the ratio by inhibiting Th1 differentiation.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 046035469

Download citation: RISBibTeXText

PMID: 8525508

DOI: 10.1097/00007890-199512150-00002


Related references

Expansion of memory TH2 cells over the TH1 cells in neonatal primed mice. Transplantation (Baltimore) 60(11): 1187-1193, 1995

Neonate-primed CD8+ memory cells rival adult-primed memory cells in antigen-driven expansion and anti-viral protection. International Immunology 18(2): 249-257, 2006

Expansion of TH2 cells and downregulation of TH1 cells in neonatal primed mice. 9TH INTERNATIONAL CONGRESS OF IMMUNOLOGY [Author] The 9th International Congress of Immunology : 606, 1995

Clonal assay for t helper cells and conditions for h 2 restricted linked cooperation between t helper cells and hapten primed b cells application to the quantitation of hemo cyanin or leishmania tropica specific t helper cells in primed mice. Journal of Immunology 127(5): 1924-1930, 1981

Immune reconstitution after bone marrow transplantation: expansion of primed/memory T cells rather than recapitulation of ontogeny. Haematologica 78(2): 137, 1993

4-1BB-mediated expansion affords superior detection of in vivo primed effector memory CD8+ T cells from melanoma sentinel lymph nodes. Clinical Immunology 137(2): 221-233, 2010

Comparison of Neonatal and Adult Mice-derived Sertoli Cells in Support of Expansion of Mouse Spermatogonial Stem Cells In vitro. International Journal of Fertility and Sterility 5(4): 217-224, 2012

Inhibition of alloantigen-primed memory CD4+ and CD8+ T cells by hematopoietic chimerism in mice. Scandinavian Journal of Immunology 72(2): 86-93, 2010

Incomplete effector/memory differentiation of antigen-primed CD8+ T cells in gene gun DNA-vaccinated mice. European Journal of Immunology 33(7): 1941-1948, 2003

Cutting edge: IL-7-dependent homeostatic proliferation of CD8+ T cells in neonatal mice allows the generation of long-lived natural memory T cells. Journal of Immunology 172(1): 15-19, 2004

Combination of antibodies inhibits accelerated rejection mediated by memory T cells in xenoantigen-primed mice. Xenotransplantation 17(6): 460-468, 2011

Drug-induced autoantibody formation in mice: triggering by primed CD4+CD25- T cells, prevention by primed CD4+CD25+ T cells. European Journal of Immunology 34(1): 36-46, 2004

IL-4 synthesis by in vivo-primed memory CD4+ T cells: II. Presence of IL-4 is not required for IL-4 synthesis in primed CD4+ T cells. Journal of Clinical Immunology 15(2): 105-115, 1995

Phenotypical and functional alterations during the expansion phase of invariant Valpha14 natural killer T (Valpha14i NKT) cells in mice primed with alpha-galactosylceramide. Immunology 116(1): 30-37, 2005

Combined costimulation blockade inhibits accelerated rejection mediated by alloantigen-primed memory T cells in mice. Immunological Investigations 38(7): 639-651, 2009