EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Exploring the folding pathways of annexin I, a multidomain protein. II. Hierarchy in domain folding propensities may govern the folding process


Exploring the folding pathways of annexin I, a multidomain protein. II. Hierarchy in domain folding propensities may govern the folding process



Journal of Molecular Biology 279(5): 1177-1185



ISSN/ISBN: 0022-2836

PMID: 9642093

DOI: 10.1006/jmbi.1998.1828

In the context of exploring the relationship between sequence and folding pathways, the multi-domain proteins of the annexin family constitute very attractive models. They are constituted of four approximately 70-residue domains, named D1 to D4, with identical topologies but only limited sequence homology of approximately 30%. The domains are organized in a pseudochiral circular arrangement. Here, we report on the folding propensity of the D1 domain of annexin I obtained from overexpression in Escherichia coli. Unlike the D2 domain, which is only partially folded, the isolated D1 domain exhibits autonomous refolding in pure aqueous solution. Similarly, the D3 domain and D2-D3 module were obtained from expression in E. coli but were found to be largely unfolded. No conclusion could be drawn for the D4 domain because it was not possible to extract it from the bacterial inclusion bodies. The data allow us to propose a plausible scenario for the annexin I folding. This working model states that firstly the D1 domain folds, and the D2 and D3 domains remain partly unfolded, facilitating the docking of the D4 domain to the D1 domain. In a second step, the D1 and D4 domains dock, and D4 may fold if already not folded. The final step starts with the stabilization of the D1-D4 module. This stabilization is crucial for allowing the non-native local interactions inside the still partially unfolded D2 domain to switch to the native long-range interactions involving D4. This switch allows the complete folding of D2 and D3. The model proposes a sequential and hierarchical process for the folding of annexin I and emphasizes the role of both native framework and non-native structures in the process.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 046043791

Download citation: RISBibTeXText

Related references

Exploring the folding pathways of annexin I, a multidomain protein. I. non-native structures stabilize the partially folded state of the isolated domain 2 of annexin I. Journal of Molecular Biology 279(5): 1163-1175, 1998

Recombination of S-peptide with S-protein during folding of ribonuclease S. I. Folding pathways of the slow-folding and fast-folding classes of unfolded S-protein. Journal of Molecular Biology 135(1): 231-244, 1979

PH jump studies of the folding of the multidomain ribosomal protein L9: the structural organization of the N-terminal domain does not affect the anomalously slow folding of the C-terminal domain. Biochemistry 39(16): 4955-4962, 2000

Transient intermediates are populated in the folding pathways of single-domain two-state folding protein L. Journal of Chemical Physics 148(16): 165101, 2018

Molecular dissection of the folding mechanism of the a subunit of tryptophan synthase: an amino-terminal autonomous folding unit controls several rate-limiting steps in the folding of a single domain protein. Biochemistry (American Chemical Society) 38(31): 205-14, 1999

Molecular dissection of the folding mechanism of the alpha subunit of tryptophan synthase: an amino-terminal autonomous folding unit controls several rate-limiting steps in the folding of a single domain protein. Biochemistry 38(31): 10205-10214, 1999

Function of Trigger Factor and DnaK in Multidomain Protein Folding: Increase in Yield at the Expense of Folding Speed. Cell 117(2): 199-209, 2004

Folding pathways of immunoglobulin domains. The folding kinetics of the Cgamma3 domain of human IgG1. Biochemistry 18(15): 3327-3336, 1979

Protein folding as a complex reaction: a two-component potential for the driving force of folding and its variation with folding scenario. Plos one 10(4): E0121640, 2015

The protein folding network indicates that the ultrafast folding mutant of villin headpiece subdomain has a deeper folding funnel. Journal of Chemical Physics 134(20): 205104, 2011

Molecular basis of co-operativity in protein folding. III. Structural identification of cooperative folding units and folding intermediates. Journal of Molecular Biology 227(1): 293-306, 1992

From folding theories to folding proteins: a review and assessment of simulation studies of protein folding and unfolding. Annual Review of Physical Chemistry 52: 499-535, 2001

Exploring the protein folding landscape Simple model study of folding dynamics. Biophysical Journal 68(2 Part 2): A122, 1995

Structural hierarchy of regulatory elements in the folding and transport of an intestinal multidomain protein. Journal of Biological Chemistry 285(6): 4143-4152, 2010

Folding of multidomain proteins: biophysical consequences of tethering even in apparently independent folding. Proteins 80(12): 2780-2798, 2012