+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Expression of a cysteine-rich protein (CRP) encoding gene during early development of the trout



Expression of a cysteine-rich protein (CRP) encoding gene during early development of the trout



Mechanisms of Development 76(1-2): 179-183



Members of the cysteine-rich protein (CRP) define a subclass of LIM-only proteins implicated mainly in muscle differentiation. Until now, very little is known concerning the expression of CRP encoding genes during vertebrate development. We describe here the isolation of a trout (Oncorhynchus mykiss) gene encoding a cysteine-rich protein (TCRP) and the pattern of its mRNA accumulation during embryogenesis, focusing on somitogenesis. TCRP encodes a putative protein with two LIM domains linked to a short glycine-rich region that displays 86%, 76%, 67% identity with chicken CRP2, CRP1 and MLP/CRP3 proteins, respectively. Whole-mount in situ hybridisation showed that TCRP transcript is first detected just before somitogenesis in the paraxial mesoderm, while it is absent in the axial structures. During somitogenesis, the expression of TCRP was observed caudally in the elongating presomitic mesoderm and in the last formed somites. The labelling for TCRP was found to fade as the somites mature. At the end of the somitogenesis, TCRP transcripts accumulation was restricted to pronephros and bronchial arches.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 046048092

Download citation: RISBibTeXText

PMID: 9867350

DOI: 10.1016/s0925-4773(98)00127-0


Related references

Structure, mapping, and expression of fisp-12, a growth factor-inducible gene encoding a secreted cysteine-rich protein. Cell Growth and Differentiation 2(5): 225-233, 1991

CRIM1, a novel gene encoding a cysteine-rich repeat protein, is developmentally regulated and implicated in vertebrate CNS development and organogenesis. Mechanisms of Development 90(2): 181-193, 2000

Preferential expression of alternatively spliced mRNAs encoding type II procollagen with a cysteine-rich amino-propeptide in differentiating cartilage and nonchondrogenic tissues during early mouse development. Developmental Biology 159(2): 403-417, 1993

Embryonic expression of the cysteine rich protein 61 (CYR61) gene: A candidate for the development of human epispadias. Birth Defects Research. Part A, Clinical and Molecular Teratology 88(7): 546-550, 2010

A new cysteine-rich protein-encoding gene family in Giardia duodenalis. Gene 169(1): 33-38, 1996

Suppression in transformed avian fibroblasts of a gene (crp) encoding a cysteine-rich protein containing LIM domains. Oncogene 8(9): 2317-2324, 1993

TTG-2, a new gene encoding a cysteine-rich protein with the LIM motif, is overexpressed in acute T-cell leukaemia with the t(11;14)(p13;q11). Oncogene 6(10): 1887-1893, 1991

The 3' terminal region of a gene encoding a cysteine-rich surface protein in Giardia duodenalis. International Journal for Parasitology 23(6): 785-792, 1993

Isolation and developmental expression of a rat cDNA encoding a cysteine-rich zinc finger protein. Nucleic Acids Research 22(24): 5477-5483, 1994

Crescent, a novel chick gene encoding a Frizzled-like cysteine-rich domain, is expressed in anterior regions during early embryogenesis. International Journal of Developmental Biology 41(3): 449-458, 1997

Ttg 2 a new gene encoding a cysteine rich protein with the lim motif is overexpressed in acute t cell leukaemia with the translocation 11 14p13 q11. Oncogene 6(10): 1887-1894, 1991

Molecular characterization of a gene encoding a cysteine-rich protein preferentially expressed in anthers of Lycopersicon esculentum. Plant molecular biology 23(3): 477-487, 1993

Cloning and expression of a gene encoding gallerimycin, a cysteine-rich antifungal peptide, from eri-silkworm, Samia cynthia ricini. Comparative Biochemistry and Physiology. Part B, Biochemistry and Molecular Biology 150(2): 229-232, 2008

Characterization of a gene encoding a cysteine-rich keratin associated protein synthesized late in rabbit hair follicle differentiation. Differentiation; Research in Biological Diversity 58(3): 227-232, 1995

Potato mop-top virus: the coat protein-encoding RNA and the gene for cysteine-rich protein are dispensable for systemic virus movement in Nicotiana benthamiana. Journal of General Virology 84(Pt 4): 1001-1005, 2003