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Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission



Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission



Proceedings of the National Academy of Sciences of the United States of America 97(15): 8206-8210



The diffraction barrier responsible for a finite focal spot size and limited resolution in far-field fluorescence microscopy has been fundamentally broken. This is accomplished by quenching excited organic molecules at the rim of the focal spot through stimulated emission. Along the optic axis, the spot size was reduced by up to 6 times beyond the diffraction barrier. The simultaneous 2-fold improvement in the radial direction rendered a nearly spherical fluorescence spot with a diameter of 90-110 nm. The spot volume of down to 0.67 attoliters is 18 times smaller than that of confocal microscopy, thus making our results also relevant to three-dimensional photochemistry and single molecule spectroscopy. Images of live cells reveal greater details.

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Accession: 046106023

Download citation: RISBibTeXText

PMID: 10899992

DOI: 10.1073/pnas.97.15.8206


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