+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Formyl-methionyl-leucyl-phenylalanine induces prostaglandin E2 release from human amnion-derived WISH cells by phospholipase C-mediated [Ca+]i rise

Formyl-methionyl-leucyl-phenylalanine induces prostaglandin E2 release from human amnion-derived WISH cells by phospholipase C-mediated [Ca+]i rise

Biology of Reproduction 64(3): 865-870

The presence of binding sites for formyl-methionyl-leucyl-phenylalanine (fMLP), its effect on prostaglandin E (PGE) release, and the signal transduction pathway activated by the peptide were investigated in human amnion-derived WISH cells. Our results demonstrate that specific binding sites for fMLP are present on WISH cells and that the peptide induces a significant increase of prostaglandin (PG)E2 release. The kinetic properties of binding are similar to those previously found in amnion tissue prior to the onset of labor, i.e., only one population of binding sites with low affinity for the peptide is present. Binding of 3H-fMLP in WISH cells is inhibited by N-t-butoxycarbonyl-methionyl-leucyl-phenylalanine, an fMLP receptor antagonist, with an IC50 value very close to that shown by nonlaboring amnion. The fMLP-induced PGE2 output is inhibited by indomethacin, quinacrine, and U-73122, inhibitors of cyclooxygenase, phospholipase A2, and phospholipase C, respectively. As regards the transduction pathway activated by fMLP, we demonstrate that phospholipase C activation, followed by an increase of intracellular calcium concentration ([Ca2+]i), is involved in response to the peptide. Our results add further evidence to the role of proinflammatory agents in the determination of labor. Furthermore, because WISH cells appear to behave like nonlaboring amnion tissue, they represent the ideal candidate for in vitro investigation of the events triggering the mechanism of delivery.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 046117059

Download citation: RISBibTeXText

PMID: 11207202

DOI: 10.1095/biolreprod64.3.865

Related references

Hemorrhage induces a reduction in the capacity of macrophages to mobilize intracellular calcium secondary to formyl-methionyl-leucyl-phenylalanine stimulation: association with alterations in cells surface Fc receptor expression and increased prostaglandin release. Shock 1(3): 228-235, 1994

Prostaglandin E2 inhibits the phospholipase D pathway stimulated by formyl-methionyl-leucyl-phenylalanine in human neutrophils. Involvement of EP2 receptors and phosphatidylinositol 3-kinase gamma. Molecular Pharmacology 66(2): 293-301, 2004

N-formyl-methionyl-leucyl-phenylalanine (fMLP) promotes osteoblast differentiation via the N-formyl peptide receptor 1-mediated signaling pathway in human mesenchymal stem cells from bone marrow. Journal of Biological Chemistry 286(19): 17133-17143, 2011

Phospholipase D activation by platelet-activating factor, leukotriene B4, and formyl-methionyl-leucyl-phenylalanine in rabbit neutrophils. Phospholipase D activation is involved in enzyme release. Journal of Immunology 146(10): 3536-3541, 1991

Standard Thermodynamic Functions of Tripeptides N -Formyl-l-methionyl-l-leucyl-l-phenylalaninol and N -Formyl-l-methionyl-l-leucyl-l-phenylalanine Methyl Ester. Journal of Chemical and Engineering Data 59(4): 1240-1246, 2014

N-formyl-methionyl-leucyl-phenylalanine induces and modulates IL-1 and IL-6 in human PBMC. Cytokine 8(6): 468-475, 1996

The N-formyl methionyl peptide, formyl-methionyl-leucyl phenylalanine (fMLF) increases the lateral diffusion of complement receptor 1 (CR1/CD35) in human neutrophils; A causative role for oxidative metabolites?. Bioscience Reports 16(5): 391-404, 1996

Fibrinogen induces IL-8 synthesis in human neutrophils stimulated with formyl-methionyl-leucyl-phenylalanine or leukotriene B(4). Journal of Immunology 167(5): 2869-2878, 2001

Superoxide anion production and phospholipase D-mediated generation of diacylglycerol are subnormal after N-formyl-methionyl-leucyl-phenylalanine stimulation of polymorphonuclear granulocytes in polycythemia vera. Journal of Laboratory and Clinical Medicine 121(2): 310-319, 1993

Exposure of N-formyl-L-methionyl-L-leucyl-L-phenylalanine-activated human neutrophils to the Pseudomonas aeruginosa-derived pigment 1-hydroxyphenazine is associated with impaired calcium efflux and potentiation of primary granule enzyme release. Infection and Immunity 67(10): 5157-5162, 1999

Cyclic ADP-ribose mediates formyl methionyl leucyl phenylalanine (fMLP)-induced intracellular Ca(2+) rise and migration of human neutrophils. Journal of Pharmacological Sciences 106(3): 492-504, 2008

Effect of ambroxol on cytosolic calcium rise in human polymorphonuclear leukocytes after stimulation with N-formyl-methionyl-leucyl-phenylalanine and concanavalin A. International Journal of Immunopathology & Pharmacology 7(1): 47-56, 1994

Phospholipase D is required in the signaling pathway leading to p38 MAPK activation in neutrophil-like HL-60 cells, stimulated by N-formyl-methionyl-leucyl-phenylalanine. Journal of Biological Chemistry 276(34): 31752-9, 2001

Investigation on the effect of experimental phospholipase A2 inhibitors on the formyl-methionyl-leucyl-phenylalanine-stimulated chemotaxis of human leukocytes in vitro. Arzneimittel-Forschung 48(1): 77-81, 1998

Interleukin-1 beta induces the synthesis and activity of cytosolic phospholipase A2 and the release of prostaglandin E2 in human amnion-derived WISH cells. Prostaglandins 49(6): 351-369, 1995