+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Further characterization of Delta(8)-sphingolipid desaturases from higher plants



Further characterization of Delta(8)-sphingolipid desaturases from higher plants



Biochemical Society Transactions 28(6): 638-641



A previously cloned cDNA from Helianthus annuus codes for a fusion protein composed of an N-terminal cytochrome b(5) and a C-terminal desaturase domain. For a functional identification, this cDNA was expressed in Saccharomyces cerevisiae and the structures of sphingolipid long-chain bases were analysed. The expression of this sunflower enzyme resulted in the formation of new Delta(8)-trans/cis-phytosphingenine from C(18)- and C(20)-phytosphinganine present in wild-type yeast cells. To elucidate the substrate specificity, the recently cloned Delta(8)-sphingolipid desaturases from Arabidopsis thaliana and Brassica napus were expressed in the yeast mutant sur2Delta that lacked the sphinganine C(4)-hydroxylase and was thus unable to form phytosphinganine. Long-chain base analysis of the transformed mutant cells did not show any conversion of C(18)- or C(20)-sphinganine into Delta(8)-sphingenine, whereas exogenously added C(18)-phytosphinganine was desaturated to Delta(8)-trans/cis-phytosphingenine. Furthermore, GLC-MS analysis did not reveal the presence of any Delta(9)-regioisomers as reported before. These results show that the sunflower gene codes for a Delta(8)-sphingolipid desaturase which accepts C(18)- and C(20)-phytosphinganine. The absence of Delta(8)-sphingenine as desaturation product in the transformed mutant suggests that C(4)-hydroxylation of sphinganine precedes Delta(8)-desaturation. Therefore, in yeast, the substrate for the plant Delta(8)-sphingolipid desaturase seems to be the phytosphinganine residue.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 046139189

Download citation: RISBibTeXText

PMID: 11171153


Related references

Further characterization of 8 -sphingolipid desaturases from higher plants. Biochemical Society Transactions 28(6): 638-641, 2000

Characterization of a Delta8-sphingolipid desaturase from higher plants: a stereochemical and mechanistic study on the origin of E,Z isomers. Angewandte Chemie 41(13): 2298-2300, 2002

Delta-8 desaturation activity varies among fatty acyl desaturases of teleost fish: high activity in delta-6 desaturases of marine species. Comparative Biochemistry and Physiology. Part B Biochemistry and Molecular Biology 159(4): 206-213, 2011

Characterization of bifunctional sphingolipid Δ4-desaturases/C4-hydroxylases of trypanosomatids by liquid chromatography-electrospray tandem mass spectrometry. Molecular and Biochemical Parasitology 184(1): 29-38, 2012

Microsomal delta 8,14-sterol delta 14-reductase in higher plants. Characterization and inhibition by analogues of a presumptive carbocationic intermediate of the reduction reaction. European Journal of Biochemistry 185(3): 605-614, 1989

Variants in CPT1A, FADS1, and FADS2 are Associated with Higher Levels of Estimated Plasma and Erythrocyte Delta-5 Desaturases in Alaskan Eskimos. Frontiers in Genetics 3: 86, 2012

Genes for microsomal delta-12 fatty acid desaturases and hydroxylases from plants. Official Gazette of the United States Patent & Trademark Office Patents 1257(3), 2002

Roles of plastid omega-3 fatty acid desaturases in defense response of higher plants. Journal of Plant Research 111(1104): 481-486, 1998

Effect of dietary trans fatty acids on the delta 5, delta 6 and delta 9 desaturases of rat liver microsomes in vivo. Acta Biologica et Medica Germanica 40(12): 1699-1705, 1981

Effect of different amino acid diets on delta 5, delta 6 and delta 9 desaturases. Lipids 19(2): 154-157, 1984

Characterization of three novel desaturases involved in the delta-6 desaturation pathways for polyunsaturated fatty acid biosynthesis from Phytophthora infestans. Applied Microbiology and Biotechnology 97(17): 7689-7697, 2013

A sphingolipid desaturase from higher plants. Identification of a new cytochrome b5 fusion protein. Journal of Biological Chemistry 273(44): 28590-6, 1998

Chimeras of Delta6-fatty acid and Delta8-sphingolipid desaturases. Biochemical and Biophysical Research Communications 279(3): 779-785, 2000

Identification of plant sphingolipid desaturases using chromatography and mass spectrometry. Journal of Chromatographic Science 47(10): 895-901, 2010

Identification and characterization of a sphingolipid delta 4-desaturase family. Journal of Biological Chemistry 277(28): 25512-8, 2002