+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Host and bacterial factors involved in the innate ability of mouse macrophages to eliminate internalized unopsonized Escherichia coli



Host and bacterial factors involved in the innate ability of mouse macrophages to eliminate internalized unopsonized Escherichia coli



Infection and Immunity 68(1): 125-132



In an effort to better understand genetic and cellular factors that influence innate immunity, we examined host and bacterial factors involved in the nonopsonic phagocytosis and killing of Escherichia coli K-12 by mouse macrophages. Unelicited (resident) peritoneal macrophages from five different mouse strains, BALB/c, C57BL/6, CD-1, C3H/HeJ, and C3H/HeN, were employed. Additional macrophage populations were obtained from CD-1 mice (bone marrow-derived macrophages). Also, for BALB/c and C57BL/6 mice, peritoneal macrophages elicited with either thioglycolate or proteose peptone, bone marrow-derived macrophages, and macrophage-like cell lines derived from the two strains were employed. Two E. coli K-12 strains that differed specifically in their abilities to produce type 1 pili containing the adhesive protein FimH were examined. The parameters used to assess macrophage bacteriocidal activity were (i) the killing of internalized (gentamicin-protected) E. coli during the approximately 4-h assay and (ii) the initial rate at which internalized E. coli were eliminated. Data on these parameters allowed the following conclusions: (i) unelicited or proteose peptone-elicited peritoneal macrophages were significantly better at eliminating internalized bacteria than thioglycolate-elicited peritoneal macrophages, bone marrow-derived macrophages, or macrophage cell lines; (ii) the host genetic background had no significant effect upon the ability of unelicited peritoneal macrophages to kill E. coli (even though the mouse strains differ widely in their in vivo susceptibilities to bacterial infection); and (iii) the FimH phenotype had no significant effect upon E. coli survival once the bacterium was inside a macrophage. Additionally, there was no correlation between the bacteriocidal effectiveness of a macrophage population and the number of bacteria bound per macrophage. However, macrophage populations that were the least bacteriocidal tended to bind higher ratios of FimH(+) to FimH(-) E. coli. The effect of gamma interferon, fetal calf serum, and the recombination proficiency of E. coli were examined as factors predicted to influence intracellular bacterial killing. These had no effect upon the rate of E. coli elimination by unelicited peritoneal macrophages.

(PDF emailed within 1 workday: $29.90)

Accession: 046274716

Download citation: RISBibTeXText

PMID: 10603378


Related references

The interaction of macrophages and bacteria escherichia coli species bacterial lipopolysaccharide and lipid a differ in their ability to induce tumoricidal activity and the secretion of reactive nitrogen intermediates in macrophages. Cellular Immunology 141(1): 47-58, 1992

Bacterial-epithelial contact is a key determinant of host innate immune responses to enteropathogenic and enteroaggregative Escherichia coli. Plos One 6(10): E27030, 2012

Role of host and bacterial virulence factors in Escherichia coli spontaneous bacterial peritonitis. European Journal of Gastroenterology and Hepatology 20(9): 924-929, 2008

Role of bacterial virulence factors and host factors in the outcome of Escherichia coli bacteraemia. European Journal of Clinical Microbiology & Infectious Diseases 19(4): 312-316, 2000

Analysis of Escherichia coli type 1 pili adhesin mutants for their ability to bind guinea pig erythrocytes and mouse peritoneal macrophages. Abstracts of the General Meeting of the American Society for Microbiology 99: 42-43, 1999

The co-transcriptome of uropathogenic Escherichia coli-infected mouse macrophages reveals new insights into host-pathogen interactions. Cellular Microbiology 17(5): 730-746, 2015

Exosomes Released from Cells Infected with Crohn's Disease-associated Adherent-Invasive Escherichia coli Activate Host Innate Immune Responses and Enhance Bacterial Intracellular Replication. Inflammatory Bowel Diseases 22(3): 516-528, 2016

Different roles of host and bacterial factors in Escherichia coli extra-intestinal infections. Clinical Microbiology and Infection 15(4): 372-379, 2009

Role of avian pathogenic Escherichia coli virulence factors in bacterial interaction with chicken heterophils and macrophages. Infection and Immunity 71(1): 494-503, 2002

Identification of bacterial factors involved in type 1 fimbria expression using an Escherichia coli K12 proteome chip. Molecular and Cellular Proteomics 13(6): 1485-1494, 2015

Bacterial adhesins and host factors: role in the development and outcome of Escherichia coli bacteremia. Clinical Infectious Diseases 17(1): 89-97, 1993

Bacteremia with escherichia coli in diabetic patients studies on bacterial virulence and host factors. Diabete & Metabolisme 14(5): 625-628, 1988

Influence of some bacterial and host factors on colonization and invasiveness of Escherichia coli K1 in neonatal rats. Infection and Immunity 61(5): 2138-2144, 1993

Relationship between the mouse colonizing ability of a human fecal Escherichia coli strain and its ability to bind a specific mouse colonic mucous gel protein. Infection and Immunity 40(1): 62-69, 1983

Bringing down the host: enteropathogenic and enterohaemorrhagic Escherichia coli effector-mediated subversion of host innate immune pathways. Cellular Microbiology 17(3): 318-332, 2015