+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Host cell specific activity of RTX toxins from haemolytic Actinobacillus equuli and Actinobacillus suis



Host cell specific activity of RTX toxins from haemolytic Actinobacillus equuli and Actinobacillus suis



Veterinary Microbiology 92(1-2): 161-167



We assessed and compared host cell specificity of the haemolytic and cytotoxic activity of the RTX toxins from Actinobacillus equuli, an equine pathogen, and Actinobacillus suis, which is pathogenic for pigs. The two bacterial species are closely related, phenotypically as well as phylogenetically, sharing the same 16S rRNA gene sequence. Both species contain specific protein toxins from the family of pore-forming RTX toxins, however, the two species differ in their RTX toxin profiles. Haemolytic A. equuli contains the operon for the Aqx toxin, whereas A. suis harbours genes for ApxI and ApxII. We tested the toxic activity of the corresponding proteins on erythrocytes as well as on lymphocytes isolated from horse and pig blood. The strength of the haemolytic activity for each of the toxins was independent of the origin of erythrocytes. When testing cytotoxic activity, the Aqx protein showed a higher toxic effect for horse lymphocytes than for porcine lymphocytes. On the other hand, ApxI and ApxII showed a strong cytotoxic effect on porcine lymphocytes and a reduced toxicity for horse lymphocytes; the toxicity of ApxII was generally much lower than ApxI. Our results indicate a host species specificity of the toxic activity of RTX toxins Aqx of A. equuli and ApxI and ApxII of A. suis.

(PDF emailed within 1 workday: $29.90)

Accession: 046274746

Download citation: RISBibTeXText

PMID: 12488079


Related references

Host cell specific activity of RTX toxins from haemolytic Actinobacillus equuli and Actinobacillus suis. Veterinary microbiology003 92(1-2): 161-167, 2003

Prevalence of organisms described as Actinobacillus suis or haemolytic Actinobacillus equuli in the oral cavity of horses. Comparative investigations of strains obtained and porcine strains of A. suis sensu stricto. Acta Pathologica, Microbiologica et Immunologica Scandinavica, B 92(6): 291-298, 1984

Reclassification of equine isolates previously reported as Actinobacillus equuli, variants of A. equuli, Actinobacillus suis or Bisgaard taxon 11 and proposal of A. equuli subsp. equuli subsp. nov. and A. equuli subsp. haemolyticus subsp. nov. International Journal of Systematic and Evolutionary Microbiology 52(Pt 5): 1569-1576, 2002

Prevalence of organisms described as actinobacillus suis or hemolytic actinobacillus equuli in the oral cavity of horses comparative investigations of strains obtained and porcine strains of actinobacillus suis sensu stricto. Acta Pathologica Microbiologica et Immunologica Scandinavica Section B Microbiology 92(6): 291-298, 1984

First Human Case of Meningitis and Sepsis in a Child Caused by Actinobacillus suis or Actinobacillus equuli. Journal of Clinical Microbiology 53(6): 1990-1992, 2016

Production of Apx toxins by field strains of Actinobacillus pleuropneumoniae and Actinobacillus suis. Infection and Immunity 62(9): 4063-4065, 1994

Reevaluation of original strains Actinobacillus suis and haemolytic strains Actinobacillus lignieresii ATCC isolated from organs of diseased pigs. Acta Univ. Agric. Fac. vet, Brno, 37: 277-290, 1968

Haemolytic strains of Actinobacillus equuli. New Zealand Veterinary Journal 20(6): 102-102, 1972

Base composition of the DNA in Pasteurella haemolytica, Actinobacillus lignieresii and Actinobacillus equuli. Zentbl. Bakt. ParasitKde. 202: 468-478, 1967

Characterisation of Australian isolates of Actinobacillus capsulatus, Actinobacillus equuli, Pasteurella caballi and Bisgaard Taxa 9 and 11. Australian Veterinary Journal 75(1): 52-55, 1997

Deoxyribonucleic acid base composition of species Pasteurella haemolytica, Actinobacillus lignieresii and Actinobacillus equuli. Zentralbl Bakteriol Parasitenk Infektionskr Hyg Erste Abt Orig 202(4): 468-478, 1967

Isolation of Actinobacillus lignieresii and Actinobacillus equuli from laboratory rodents. Journal of Clinical Microbiology 12(3): 351-354, 1980

A haemolytic variant of Actinobacillus equuli causing an acute septicaemia in a foal. New Zealand Veterinary Journal 19(11): 264-265, 1971