+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Host nuclear abnormalities and depletion of nuclear antigens induced in Trichinella spiralis-infected muscle cells by the anthelmintic mebendazole

Host nuclear abnormalities and depletion of nuclear antigens induced in Trichinella spiralis-infected muscle cells by the anthelmintic mebendazole

Molecular and Biochemical Parasitology 96(1-2): 1-13

Infection by the parasitic nematode Trichinella spiralis induces cell cycle repositioning (chronic suspension in apparent G2/M) and genetic reprogramming in differentiated mammalian skeletal muscle cells. These changes occur in association with dramatic enlargement of infected host cell nuclei (as large as 17 microm in diameter) and nucleoli. Nuclear antigens (NA) that colocalize with host chromatin have been detected by antibodies to T. spiralis antigens, but the functions of these NA are unresolved. Mebendazole (MBZ) preferentially binds parasite versus host beta-tubulins, is implicated in inhibiting secretion in nematodes and induces cytoplasmic changes in muscle cells infected with T. spiralis. These infected cell changes might be indirect via MBZ inhibition of parasite secretions. This effect would have implications for host/parasite interactions and was evaluated here. MBZ treatment of chronically infected mice caused: (1) a significant deformation of host nuclei and diminution of nucleoli by 4 and 6 days of treatment (dot), respectively; (2) a reduction of nuclear lamins A/C in infected cell nuclei that was concomitant with nuclear deformation; and (3) significant reductions in total RNA, general protein and acid phosphatase activity levels. These changes were associated with the depletion of NA from host nuclei detected by 4 dot. However, DNA content of infected cell nuclei was not detectably reduced and muscle gene expression was not reactivated. The cellular changes documented are likely to account for previously described cytoplasmic alterations induced by MBZ. Concomitant depletion of NA from infected cell nuclei suggests a role of these products in regulating nuclear functions of host cells.

(PDF emailed within 0-6 h: $19.90)

Accession: 046274861

Download citation: RISBibTeXText

PMID: 9851602

DOI: 10.1016/s0166-6851(98)00082-6

Related references

Nuclear antigens in Trichinella spiralis infected muscle cells: nuclear extraction, compartmentalization and complex formation. Molecular and Biochemical Parasitology. 1 May; 922: 207-218, 1998

Trichinella spiralis-infected muscle cells: abundant RNA polymerase II in nuclear speckle domains colocalizes with nuclear antigens. Infection and Immunity 69(6): 4065-4071, 2001

Host immune response to parasitizing of trichinella and its changes under the effect of mebendazole part 2 cellular and humoral immune responses in mice infected with trichinella spiralis after mebendazole therapy. Meditsinskaya Parazitologiya i Parazitarnye Bolezni 47(6): 23-28, 1978

Host immune response to Trichinella parasitization and its alteration under mebendazole exposure. II. The cellular and humoral immune reactions of mice infected with Trichinella spiralis after mebendazole treatment. Meditsinskaia Parazitologiia i Parazitarnye Bolezni 47(6): 23-28, 1978

Use of ELISA and Western blot for serological detection of antibodies to E-S antigens of Trichinella spiralis muscle larvae in sera of swine experimentally infected with Trichinella spiralis. Veterinary Immunology and Immunopathology 203: 13-20, 2018

The use of nuclear magnetic resonance nmr to study tissues of mice infected with trichinella spiralis and trichinella pseudospiralis. Acta Parasitologica Polonica 33(3): 209-214, 1988

Anthelmintic and histopathological effects of mebendazole on Trichinella spiralis in the rat. Trichinellosis Proc 3rd Int Conf on Trichinellosis, Florida, USA, 2-4 Nov 1972: 515-527, 1974

Failure to detect Trichinella spiralis p43 in isolated host nuclei and in irradiated larvae of infected muscle cells which express the infected cell phenotype. Molecular and Biochemical Parasitology 67(2): 225-234, 1994

The anthelmintic action of benzimidazole carbamates on Trichinella spiralis. I. Mebendazole. Revista Iberica de Parasitologia 38(1/2): 249-258, 1978

Efficacy of albendazole, oxibendazole, oxfendazole and mebendazole against muscle larvae of Trichinella spiralis in experimentally infected pigs. Chinese Journal of Veterinary Science and Technology (5): 12-15, 1987

Anthelmintic effects of albendazole, mebendazole and diethylcarbamazine on Trichinella spiralis in mice. Journal of the Science Society of Thailand 15(1): 49-54, 1989

Phosphocholine antigens in the muscle larvae of Trichinella spiralis Antygeny fosfocholinowe larw inwazyjnych Trichinella spiralis. Wiadomosci Parazytologiczne, 421: 57-63, 1996

Study of the reaginic response originated by trichinella spiralis and trichinella pseudospiralis after antihelmintic treatment of muscle larvae with mebendazole. Revista Iberica de Parasitologia 48(1): 101-108, 1988

Light and electron microscopic observation of murine muscle cells infected with Trichinella spiralis and Trichinella pseudospiralis. Trichinellosis Proceedings of the Seventh International Conference on Trichinellosis, Alicante, Spain, 2-6 October 1988: 304-311, 1989

Analysis of the diagnostic efficiency of excretory-secretory antigens of Trichinella spiralis and Trichinella nativa with sera from rats experimentally infected with arctic Trichinella, by using ELISA. Meditsinskaia Parazitologiia i Parazitarnye Bolezni 2012(3): 14-17, 2012