+ Translate

Identification of a second murine interleukin-11 receptor alpha-chain gene (IL11Ra2) with a restricted pattern of expression

, : Identification of a second murine interleukin-11 receptor alpha-chain gene (IL11Ra2) with a restricted pattern of expression. Genomics 40(3): 387-394

The interleukin-11 receptor alpha-chain, a member of the hematopoietin receptor superfamily, forms, together with gp130, a functional high-affinity receptor complex for interleukin 11. We, and others, reported the cloning of the murine interleukin 11 receptor alpha-chain cDNA (IL11Ra) and recently described the structure of the IL11Ra locus. We also described the presence of a second IL11Ra-like locus in some mouse strains. In this study we report that the second locus, designated IL11Ra2, encodes an mRNA species. The transcript was 99% identical to the IL11Ra transcript in the coding and 3'-untranslated region, but had a different 5'-untranslated region. The complete genomic organization of the IL11Ra2 locus is presented, and the two loci are shown to be located on a 200-kb NaeI genomic fragment. Comparison of the expression pattern of the IL11Ra and IL11Ra2 genes using an RT-PCR restriction fragment length polymorphism strategy revealed that while the expression of IL11Ra was widespread, expression of IL11Ra2 was restricted to testis, lymph node, and thymus.

(PDF same-day service: $19.90)

Accession: 046313884

PMID: 9073505

DOI: 10.1006/geno.1996.4579

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Robb, L.; Hilton, D.J.; Brook Carter, P.T.; Begley, C.G., 1997: Identification of a second murine interleukin-11 receptor alpha -chain gene (IL11Ra2) with a restricted pattern of expression. The identification and cloning of a mouse interleukin-11 receptor alpha -chain (IL11Ra) has been reported recently. In this study, the presence of a 2nd IL11Ra-like locus in some mouse strains was confirmed and the nucleotide sequence of a cDNA wa...

Hémar, A.; Dautry-Varsat, A., 1990: Cyclosporin A inhibits the interleukin 2 receptor alpha chain gene transcription but not its cell surface expression: the alpha chain stability can explain this discrepancy. The effect of the immunosuppressor cyclosporin A (CsA) on the expression of interleukin (IL) 2 receptors was investigated in a human T cell line IARC301 which constitutively expressed such receptors. This cell line also spontaneously secretes IL2...

Suzuki H.; Chung F.; Palmer E.; Sasaki T.; Ohara N.; Taylor B.A.; Ohara J., 1991: Gene mapping of murine interleukin 4 receptor il 4r the loci of the il 4r gene and lymphocyte function associated antigen 1 lfa 1 alpha chain gene are closely linked on chromosome 7. FASEB Journal 5(5): A970

Riedy, M.C.; Stewart, C.C., 1992: Quantitative reverse transcription in combination with the polymerase chain reaction for measuring gene expression of C-FGR, interleukin-6 ligand and interleukin-6 receptor in murine macrophages. Journal of Leukocyte Biology 0(SUPPL 3): 33

Davidson, A.J.; Freeman, S.A.; Crosier, K.E.; Wood, C.R.; Crosier, P.S., 1997: Expression of murine interleukin 11 and its receptor alpha-chain in adult and embryonic tissues. Interleukin 11 (IL-11) is a multifunctional cytokine that has diverse effects on blood cells and their precursors and on a number of cell types outside of the hematopoietic system. The cDNAs encoding murine EL-11 and its receptor alpha-chain (IL-1...

Nandurkar, H.H.; Robb, L.; Nicholl, J.K.; Hilton, D.J.; Sutherland, G.R.; Begley, C.G.enn, 1997: The gene for the human interleukin-11 receptor alpha chain locus is highly homologous to the murine gene and contains alternatively spliced first exons. The gene for the murine interleukin-11 receptor alpha chain (mIL-11R-alpha) contains two loci (1 and 2), of which locus 2 is restricted to only some mouse strains. Two alternatively spliced exons (1a and lb) encode the 5' untranslated region...

Sehajpal P.; Skolnik E.; Sharma V.; Wieder K.J.; Walz G.; Zanker B.; Strom T.B.; Suthanthiran M., 1990: Differential regulation of interleukin 2 receptor il 2r alpha chain and il 2r beta chain gene expression in normal human t cells demonstration by sequential reverse transcription and polymerase chain reaction. Kidney International 37(1): 599

Robb, L.; Hilton, D.J.; Willson, T.A.; Begley, C.G., 1996: Structural analysis of the gene encoding the murine interleukin-11 receptor alpha-chain and a related locus. In this study the gene for the murine interleukin-11 receptor a chain (IL-11R-alpha) has been characterized. The gene spans 9 kilobase pairs of DNA, and the organization of its 14 exons conforms to the pattern observed for other members of the hem...

Honjo, E.; Shoyama, Y.; Tamada, T.; Shigematsu, H.; Hatanaka, T.; Kanaji, S.; Arima, K.; Ito, Y.; Izuhara, K.; Kuroki, R., 2008: Expression of the extracellular region of the human interleukin-4 receptor alpha chain and interleukin-13 receptor alpha1 chain by a silkworm-baculovirus system. The receptor binding to interleukin (IL)-13 is composed of the IL-13 receptor alpha1 chain (IL-13Ralpha1) and the IL-4 receptor alpha chain (IL-4Ralpha). In order to investigate the interaction of IL-13 with IL-13Ralpha1 and IL-4Ralpha, the DNA fr...

Matsui, K.; Arai, T., 1995: Immunosuppression induced by Salmonella infection is correlated with augmentation of interleukin-2 receptor alpha chain expression in murine splenic lymphocytes. In a previous study, we observed that the suppression of T-cell proliferation induced by Salmonella cell-free extract was associated with augmentation of IL-2 receptor (IL-2R) alpha chain expression. In this study, we also observed this kind of au...