+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Identification of genomic differences between Escherichia coli strains pathogenic for poultry and E. coli K-12 MG1655 using suppression subtractive hybridization analysis



Identification of genomic differences between Escherichia coli strains pathogenic for poultry and E. coli K-12 MG1655 using suppression subtractive hybridization analysis



Microbial Pathogenesis 33(6): 289-298



Diseases of poultry caused by Escherichia coli result in significant economic loss every year. Specific virulence factors associated with E. coli strains pathogenic for poultry have been identified, but it is likely that others remain to be identified. To identify unique DNA fragments associated with avian strains we used suppression subtractive hybridization. The genome of E. coli K-12 strain MG1655 was subtracted from the genomes of two avian E. coli strains resulting in the identification of 62 fragments specific to the two avian strains. Sequence homology analysis was done and four types of fragments were identified: plasmid sequences, phage sequences, sequences with known function and sequences without any currently known function. Two E. coli collections, a reference collection of diverse strains (ECOR) and a collection of 41 avian isolates, were screened for the presence of 25 of the 62 fragments. We identified nine fragments present in significantly more of the avian strains than of the ECOR strains. Five fragments were in significantly more of the ECOR strains than the avian strains. These results suggested that the nine fragments could play a role in the pathogenesis of E. coli as it relates to diseases of poultry.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 046315545

Download citation: RISBibTeXText

PMID: 12495675

DOI: 10.1006/mpat.2002.0536


Related references

Identification of genomic differences in avian pathogenic Escherichia coli using suppression subtractive hybridization analysis. Wei Sheng Wu Xue Bao 45(5): 680-684, 2005

Unique DNA sequences of avian pathogenic Escherichia coli isolates as determined by genomic suppression subtractive hybridization. Fems Microbiology Letters 262(2): 193-200, 2006

A subtractive hybridisation analysis of genomic differences between the uropathogenic E. coli strain 536 and the E. coli K-12 strain MG1655. FEMS Microbiology Letters 199(1): 61-66, 2001

Suppression subtractive hybridization identifies an autotransporter adhesin gene of E. coli IMT5155 specifically associated with avian pathogenic Escherichia coli (APEC). Bmc Microbiology 10: 236, 2010

Common and specific genomic sequences of avian and human extraintestinal pathogenic Escherichia coli as determined by genomic subtractive hybridization. Bmc Microbiology 7: 81, 2007

A suppression subtractive hybridization analysis of genomic differences among strains of Yersinia pestis. Zhonghua Weishengwuxue He Mianyixue Zazhi 25(3): 179-182, 2005

Identification of novel virulence-associated loci in uropathogenic Escherichia coli by suppression subtractive hybridization. Fems Microbiology Letters 230(2): 203-208, 2004

XbaI and BlnI genomic cleavage maps of Escherichia coli K-12 strain MG1655 and comparative analysis of other strains. Journal of Molecular Biology 232(2): 419-445, 1993

Genomic comparison of plant pathogenic and non-pathogenic Serratia marcescens strains using suppression subtractive hybridization. Phytopathology 93(6 Suppl.): S94, 2003

Extensive genomic diversity in pathogenic Escherichia coli and Shigella Strains revealed by comparative genomic hybridization microarray. Journal of Bacteriology 186(12): 3911-3921, 2004

Identification and localization of differences between Escherichia coli and Salmonella typhimurium genomes by suppressive subtractive hybridization. Molecular & General Genetics 262(4-5): 721-729, 1999

Identification and localization of differences between Escherichia coli and Salmonella typhimurium genomes by suppressive subtractive hybridization. Molecular and General Genetics 262(4-5): 721-729, 2000

Identification of genomic differences between strains of Yersinia enterocolitica and Yersinia pseudotuberculosis by suppression subtractive hybridization. Abstracts of the General Meeting of the American Society for Microbiology 99: 256, 1999

Sequencing and functional annotation of avian pathogenic Escherichia coli serogroup O78 strains reveal the evolution of E. coli lineages pathogenic for poultry via distinct mechanisms. Infection and Immunity 81(3): 838-849, 2013

High frequency of hybrid Escherichia coli strains with combined Intestinal Pathogenic Escherichia coli (IPEC) and Extraintestinal Pathogenic Escherichia coli (ExPEC) virulence factors isolated from human faecal samples. Bmc Infectious Diseases 18(1): 544, 2018