+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Immunohistochemical characterization of hepatic lymphocytes in acute hepatitis A, B, and C

Immunohistochemical characterization of hepatic lymphocytes in acute hepatitis A, B, and C

Journal of Clinical Gastroenterology 23(3): 199-202

The distinctive histologic findings in acute hepatitis A, B, and C suggest that different immunological mechanisms are involved in the pathogenesis of these diseases. This study was undertaken to define the immune response in each type of acute hepatitis by identification of the intrahepatic lymphocyte subsets. Thirty paraffin-embedded liver biopsy specimens from 10 patients with acute hepatitis A, 10 patients with acute hepatitis B, and 10 patients with acute hepatitis C were evaluated. Immunohistochemical staining was performed by the indirect immunoperoxidase technique using the following monoclonal or polyclonal antibodies: CD45RO, CD20-cy, CD57, and Mac387. Inflammatory infiltrates varied from specimen to specimen. However, CD45RO+ memory T cells were the predominant infiltrating mononuclear cells in all specimens. In the portal areas, CD45RO+ memory T cells were the most prominent in AHC, followed by AHA and AHB, and the difference between AHC and AHB was statistically significant. CD20-cy+ B cells were seen mainly in the portal areas, and were significantly less common in AHB than in AHA and AHC. In addition, the ratio of CD20-cy+ B cells to CD45RO+ memory was significantly lower in AHB than in the other types of acute hepatitis. The necrotic areas in all specimens contained mainly CD45RO+ memory cells in association with a few CD57+ NK and T cells or CD20-cy+ B cells. Our study revealed differences of the intrahepatic lymphocyte subsets among the various types of acute hepatitis, but the meaning of these differences is presently unknown. Therefore, further studies are required to fully elucidate the mechanism of the immune response in acute hepatitis.

(PDF emailed within 1 workday: $29.90)

Accession: 046333837

Download citation: RISBibTeXText

PMID: 8899501

Related references

Immunohistochemical characterization of hepatic lymphocytes in primary biliary cirrhosis in comparison with primary sclerosing cholangitis and autoimmune chronic active hepatitis. O Clinic Proceedings. 68(11): 1049-1055, 1993

Immunohistochemical characterization of T-lymphocytes subsets in Egyptian hepatic schistosomiasis. Journal of the Egyptian Society of Parasitology 21(1): 195-202, 1991

Lymphocytotoxins in acute and chronic hepatitis. Characterization and relationship to changes in circulating T lymphocytes. Clinical and Experimental Immunology 26(1): 21-27, 1976

CD1d-restricted non-invariant T lymphocytes in hepatitis C A novel population of intra-hepatic lymphocytes. Journal of Hepatology 38(Supplement 2): 205-206, 2003

Hepatic histologic and immunohistochemical changes in chronic hepatitis B after prolonged clearance of hepatitis B e antigen and hepatitis B surface antigen. Annals of Internal Medicine 115(2): 113-115, 1991

Different quasi-species of hepatitis C virus infect peripheral lymphocytes, intra-hepatic lymphocytes and hepatocytes. Hepatology 22(4 PART 2): 222A, 1995

Characterization of sporadic acute hepatitis E and comparison of hepatitis E virus genomes in acute hepatitis patients and pig liver sold as food in Mie, Japan. Hepatology Research 44(10): E63-E76, 2013

Hepatic stellate cell activation and hepatic fibrosis in children with type 1 autoimmune hepatitis: an immunohistochemical study of paired liver biopsies before treatment and after clinical remission. European Journal of Gastroenterology and Hepatology 22(3): 264-269, 2010

Immunohistochemical characterization of the hepatic progenitor cell compartment in medaka (Oryzias latipes) following hepatic injury. Journal of Comparative Pathology 149(4): 434-445, 2014

Immunohistochemical and ultrastructural study of hepatic sinusoidal linings during dichloropropanol-induced acute hepatic necrosis. Liver. 14(2): 90-97, 1994

Characterization of CD4, CD8, CD56 positive lymphocytes and C4d deposits to distinguish acute cellular rejection from recurrent hepatitis C in post-liver transplant biopsies. Clinical Transplantation 20(5): 624-633, 2006

Distinctive intra hepatic distribution of hepatitis b surface and hepatitis b core antigens in patients with acute and chronic hepatitis b. Digestion 14(5/6): 459, 1976

Studies on the Relationship of the Hepatitis Virus to Persistent Symptoms, Disability, and Hepatic Disturbance ("chronic Hepatitis Syndrome") Following Acute Infectious Hepatitis. Journal of Clinical Investigation 26(2): 329-338, 1947

Localization of thymus derived lymphocytes and bone marrow derived lymphocytes in hepatic biopsies from patients with chronic active hepatitis. Folia Allergologica et Immunologica Clinica 25(2): 127-131, 1978

Immunological detection of hepatitis b antigen and hepatitis b antigen bonding to lymphocytes in acute and chronic hepatitis clinical personnel and healthy controls. Zeitschrift fuer Immunitaetsforschung Experimentelle und Klinische Immunologie 147(4): 328, 1974