+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Immunohistochemical detection of P53 overexpression in paraffin wax-embedded squamous cell carcinomas of cattle, horses, cats and dogs

Immunohistochemical detection of P53 overexpression in paraffin wax-embedded squamous cell carcinomas of cattle, horses, cats and dogs

Journal of Comparative Pathology 114(2): 205-210

One hundred and six squamous cell carcinomas (SCCs) of cattle, horses, cats and dogs were analysed immunohistochemically for overexpression of p53 protein. The monoclonal antibody pAb 240, which recognizes only mutant p53, was used. Of 41 bovine ocular SCCs, 26 (63.4%) showed p53 nuclear reactivity. All of six (100%) equine ocular SCCs and seven of nine (77.7%) SCCs of the equine penis or vulva gave positive reactions. In nine of 11 (81.8%) feline SCCs of the ear and in seven of 14 (50%) feline SCCs of other locations, p53 immunoreactivity was detected. Only seven of 25 (29.5%) canine cutaneous SCCs gave a positive reaction. Thus p53 antigen could be detected immunohistochemically in formalin-fixed and paraffin wax-embedded tissues of SCCs of domestic animals. The results support the view that, as in man, p53 overexpression plays an important role in the development of most SCCs of the animal species studied. This was in particular true for feline SCCs of the ear and for bovine and equine ocular SCCs, which are assumed to be related to ultraviolet radiation.

(PDF emailed within 0-6 h: $19.90)

Accession: 046333978

Download citation: RISBibTeXText

PMID: 8920221

DOI: 10.1016/S0021-9975(96)80010-7

Related references

Detection of novel papillomaviruslike sequences in paraffin-embedded specimens of invasive and in situ squamous cell carcinomas from cats. American Journal of Veterinary Research 67(12): 2036-2041, 2006

Detection of human papillomavirus DNA in squamous bronchial metaplasia and squamous cell carcinomas of the lung by in situ hybridization using biotinylated probes in paraffin-embedded specimens. Human Pathology 21(1): 111-116, 1990

Epidermal growth factor receptor expression in squamous cell lung carcinomas: An immunohistochemical and gene analysis in formalin-fixed, paraffin-embedded material. Virchows Archiv A Pathological Anatomy and Histopathology 423(4): 295-302, 1993

Immunohistochemical detection of p21-WAF1/C-IP1 and p53 proteins in formalin-fixed paraffin-embedded tissue sections of squamous cell carcinoma of the skin. Journal Of Dermatological Science. 14(3): 233-239, 1997

Detection of human papillomavirus type 16 DNA in cervical swabs and formalin-fixed, paraffin-embedded squamous cell carcinomas of non-genital tissues using a synthetic oligodeoxynucleotide probe. Journal of Virological Methods 25(3): 325-336, 1989

Immunohistochemical detection of p53 overexpression in formalin-fixed, paraffin-embedded sections of endometrial carcinoma. Journal of Obstetrics and Gynaecology 21(6): 593-600, 1995

Detection of p53 overexpression in routinely paraffin-embedded tissue of human carcinomas using a novel target unmasking fluid. American Journal of Pathology 142(2): 381-385, 1993

Immunohistochemical detection of DNA topoisomerase I in formalin fixed, paraffin wax embedded normal tissues and in ovarian carcinomas. Molecular Pathology 50(5): 247-253, 1998

Immunohistochemical detection of p185 product, p21 product, and proliferating cell nuclear antigen (PCNA) in formalin-fixed, paraffin-embedded tissues from ovarian carcinomas. Preliminary data. European Journal of Gynaecological Oncology 15(5): 359-368, 1994

DNA-measurements on formalin-fixed, paraffin-embedded squamous cell carcinomas from different ENT-regions. Pathology, Research and Practice 181(2): 230-235, 1986

Improved method for release of cell nuclei from paraffin-embedded cell material of squamous cell carcinomas. Cytometry 14(8): 931-935, 1993

Loss of heterozygosity in disaggregated nuclei from paraffin embedded squamous cell carcinomas of the uterine cervix. Proceedings of the American Association for Cancer Research Annual Meeting 38(0): 514-515, 1997

Improved immunohistochemical detection of p53 protein in paraffin--embedded tissues reveals elevated levels in most head and neck and lung carcinomas: correlation with clinicopathological parameters. Anticancer Research 12(5): 1389-1394, 1992