+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

In vitro interaction of coronaviruses with primate and human brain microvascular endothelial cells



In vitro interaction of coronaviruses with primate and human brain microvascular endothelial cells



Advances in Experimental Medicine and Biology 380: 79-88



Primary human and primate brain microvascular endothelial cells were tested for permissiveness to coronaviruses JHM and 229E. While sub-genomic viral RNAs could be detected up to 72 hours post-infection, primate cells were abortively infected and neither virus caused cytopathology. Human cells were non-permissive for JHM but permissive for 229E replication; peak production of progeny 229E and observable cytopathic effects occurred approximately 22 and 32 hour post-infection, respectively. Using the criterion of cytopathology induction in infected endothelial cells, 229E was compared to other human RNA and DNA viruses. In addition, virus induced modulation of intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1) and HLA I was monitored by immunostaining of infected cells.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 046362651

Download citation: RISBibTeXText

PMID: 8830550


Related references

Interaction of Leptospira interrogans with cultured epithelial cells. Abstracts of the General Meeting of the American Society for Microbiology 93: 126, 1993

HIV-1 infection of human CD4+ T cells in vitro. Differential induction of apoptosis in these cells. Journal of Immunology 152(1): 330-342, 1994

Interaction of SeqA and Dam methylase on the hemimethylated origin of Escherichia coli chromosomal DNA replication. Journal of Biological Chemistry 274(17): 11463-8, 1999

Interaction of Ler at the LEE5 (tir) operon of enteropathogenic Escherichia coli. Infection and Immunity 71(1): 384-392, 2003

Balaju industrial district pollution control study. 1992

NleH effectors interact with Bax inhibitor-1 to block apoptosis during enteropathogenic Escherichia coli infection. Proceedings of the National Academy of Sciences of the United States of America 107(7): 3129-3134, 2010

An in vitro model of squamous metaplasia of bronchial epithelium. Proceedings of the American Association for Cancer Research Annual Meeting (41): 458, 2000

Calcitonin gene-related peptide induced vasodilation depends on the release of nitric oxide. European Heart Journal 20(ABSTR Suppl. ): 132, 1999

A self-appraisal survey for nutrition committees. 1944

In vitro assessment of cytogenetic and oxidative effects of α-pinene. Toxicology and Industrial Health 32(1): 168-176, 2016

Approaches of extension specialists to teaching community and economic development. Journal of the Community Development Society6(1): 125-137, 1995

Escherichia coli K-12 uridine phosphorylase protein engineering. The role of cysteine residues in enzyme functioning. Molecular Biology 30: 6-10, 1996

Thrombomodulin mutations in atypical hemolytic-uremic syndrome. New England Journal of Medicine 361(4): 345-357, 2009

In vitro characterization of potential CYP- and UGT-derived metabolites of the psychoactive drug 25B-NBOMe using LC-high resolution MS. Drug Testing and Analysis 8(2): 248-256, 2016