+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

In vivo search for butyrate responsive sequences using transgenic mice carrying A gamma gene promoter mutants

In vivo search for butyrate responsive sequences using transgenic mice carrying A gamma gene promoter mutants

Blood 88(3): 1079-1083

ISSN/ISBN: 0006-4971

PMID: 8704217

DOI: 10.1182/blood.v88.3.1079.1079

We describe an in vivo approach, in transgenic mice, aimed to identify promoter elements responsible for the induction of gamma globin expression by butyrate. Transgenic lines carrying human A gamma gene promoter truncations at position -141, -201, -382, and -730 A gamma were treated with alpha amino butyric acid (alpha ABA), and effects on gamma globin expression were analyzed at the messenger RNA level. No induction of gamma gene expression was observed in animals carrying promoters truncated at positions -141, -201, or -382 A gamma, suggesting either that butyrate response elements (BRE) are not located in the proximal gamma gene promoter or, if they were, they require the cooperation of upstream sequences for gamma gene induction. Two animals from one line carrying the -730 A gamma truncation responded to alpha ABA treatment with significant increases in gamma gene expression, indicating that a BRE is located between position -382 and -730 region of the A gamma gene promoter. Because the maximum induction by alpha ABA is observed in transgenic mice carrying a A gamma gene promoter extending to nucleotide -1350, it is likely that another butyrate responsive element is located between -730 and -1350 of the A gamma gene promoter. These results indicate that the transgenic mouse model can be used for identification of DNA regions that contain cis elements involved in gamma globin gene inducibility.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 046366990

Download citation: RISBibTeXText

Related references

Search for butyrate response sequences in they A-gamma gene promoter. Blood 84(10 Suppl. 1): 364A, 1994

In vivo analysis of the phenobarbital-responsive rat CYP2B2 gene promoter and PBRU in transgenic mice. FASEB Journal 16(4): A180, 2002

Human cholesteryl ester transfer protein gene proximal promoter contains dietary cholesterol positive responsive elements and mediates expression in small intestine and periphery while predominant liver and spleen expression is controlled by 5'-distal sequences. Cis-acting sequences mapped in transgenic mice. Journal of Biological Chemistry 271(50): 31831-31838, 1996

Expression of thyroid hormone receptor betaA gene assayed by transgenic Xenopus laevis carrying its promoter sequences. Molecular and Cellular Endocrinology 181(1-2): 97-110, 2001

Male hypogonadism in transgenic mice carrying a hgr promoter c myc fusion gene. Journal of Cellular Biochemistry Suppl. (13 Part B): 173, 1989

Generation of transgenic mice carrying the E coli LacZ gene driven by a Drosophila melanogaster patched gene promoter. Biotecnologia Aplicada 13(1): 32, 1996

A strain of transgenic mice carrying an extra Mu IFN-B gene placed under the control of a constitutive promoter. Journal of Interferon Research 13(Suppl. 1): S119, 1993

Generation of transgenic mice carrying the hiv 1 nef gene under control of a t cell specific promoter. Journal of Cellular Biochemistry Suppl. (16 Part C): 144, 1992

Characterization of transgenic mice carrying the glycerol-3-phosphate dehydrogenase promoter-luciferase gene. Developmental Neuroscience 17(3): 199, 1995

On the mechanism of gamma gene induction by butyrate Role of the LCR and evidence that butyrate cannot reactivate in vivo silenced gamma genes. Blood 82(10 Suppl. 1): 312A, 1993

Neural and facial abnormalities in transgenic mice carrying the connexin 43 gene driven by a pan-active promoter. Molecular Biology of the Cell 5(Suppl. ): 326A, 1994

Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter. Proceedings of the National Academy of Sciences of the United States of America 91(20): 9302-9306, 1994

Reporter gene expression in cones in transgenic mice carrying bovine rhodopsin promoter/lacZ transgenes. Visual Neuroscience 11(6): 1227-1231, 1994

A Non-Transformed Multipotential Stem Cell Line From Transgenic Mice Carrying The Human BCL-2 Gene Under The NSE Promoter Control. Society for Neuroscience Abstracts 21(1-3): 286, 1995

Sodium butyrate-mediated induction of the glycoprotein hormone alpha-subunit gene: requirement for continued protein synthesis, identification of a butyrate-responsive element, and inhibition of promoter activation by 2-deoxyglucose. Journal of Cellular Biochemistry 74(2): 242-263, 1999