+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Influence of selenium-rich rice on transformation of umbilical blood B lymphocytes by Epstein-Barr virus and Epstein-Barr virus early antigen expression

Influence of selenium-rich rice on transformation of umbilical blood B lymphocytes by Epstein-Barr virus and Epstein-Barr virus early antigen expression

Ai Zheng 22(1): 26-29

Selenium (Se), an antioxidant, is an essential trace element to human body. It can be used as an anti-aging agent and a tumor cell proliferation inhibitor. To further investigate the effect of selenium in cancer prevention, the authors observed the influence of Se-rich rice extract on the transformation of umbilical blood B lymphocytes stimulated by Epstein-Barr virus (EBV) and expression of EBV early antigen(EBV-EA) in Raji cells. (1) Se-rich rice and general rice extract (dilution of 1:4 or 1:8) were added to mixture of EBV, and then umbilical blood mononuclear cells were added. Lymphoblasts transformation test was then performed. The inhibition rate of B lymphocytes transformation was calculated. (2) Raji cells stimulated by butyrate and croton oil were incubated with Se-rich rice extract. The EBV-EA positive expression rate and the inhibition rate were counted using indirect immunological flurescence method. The transformation of umbilical blood B lymphocytes stimulated by EBV was significantly inhibited by Se-rich rice extract at a concentration of 0.11 g/ml (1:8 diluted). The inhibition rate was 83.4% (P < 0.01), which was significantly higher than that of the control rice (63.1%) (P < 0.05). Se-rich rice extract showed significant inhibition on EBV-EA in Raji cells. As the extract concentration was at 0.016 microgram/ml, 0.078 g/ml, and 0.388 microgram/ml, the inhibition rates of EA were 2.85%, 12.88%, and 20.75%, respectively. The transformation of umbilical blood B lymphocytes stimulated by EB virus and expression of EBV-EA in Raji cells may be significantly inhibited by Se-rich rice extract, suggesting that Se-rich rice can be used for preventing nasopharyngeal carcinoma.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 046401328

Download citation: RISBibTeXText

PMID: 12561431

Related references

Effects of S-adenosyl-methionine on plasma norepinephrine, blood pressure, and heart rate in healthy volunteers. Psychiatry Research 17(2): 111-118, 1986

Phosphono formate inhibition of avian myeloblastosis virus dna polymerase. Federation Proceedings 39(6): ABSTRACT 1846, 1980

Relationship between the amino acid spectrum of brain structures and the retrieval of memory engrams under experimental neurosis. Vestnik Akademii Meditsinskikh Nauk SSSR (9): 54-57, 1985

Transfer of the conjugal tetracycline resistance transposon tn 916 from streptococcus faecalis to staphylococcus aureus and identification of some insertion sites in the staphylococcal chromosome. Journal of Bacteriology 169(5): 2121-2131, 1987

Transfection of ej h a into keratinocytes derived from carcinogen induced mouse papillomas causes malignant progression. Journal of Cellular Biochemistry Suppl. (10 Part C): 151, 1986

Analysis of epr spectra of irradiated liver and hepatoma specimens. Biofizika 23(5): 852-858, 1978

Effects of nucleoside analogs and sodium butyrate on recovery from potentially lethal X ray damage in human colon tumor cells. International Journal of Radiation Oncology Biology Physics 13(4): 593-601, 1987

Expression of epstein barr nuclear antigen in mouse cells following gene transfer. Clinical Research 30(2): 519A, 1982

Establishment of epithelial cell line mda mb 157 from metastatic pleural effusion of human breast carcinoma. In Vitro (Rockville) 9(4): 239-245, 1974

Transformation of human cells with damaged plasmids. Uicc (Union Internationale Contre Le Cancer, International Union Against Cancer) 14th International Cancer Congress, Budapest, Hungary, Aug 21-27, Abstracts, Lectures, Symposia And Free Communications, Vols 1, 2, 3, Late Abstracts, And Register Xvi+479p (Vol 1); Xvi+298p (Vol 2); Xvi+531p (Vol 3); 15p (Late Abstracts); 40p (Register) S Karger Ag: Basel, Switzerland; New York, N Y , Usa; Akademiai Kiado: Budapest, Hungary Paper 485, 1986

Epstein-Barr virus integration in human lymphomas and lymphoid cell lines. Cancer 70(1): 185-191, 1992

Immortalization of oral epithelial cells with HPV type 16. Journal of Dental Research 72(ABSTR SPEC ISSUE): 116, 1993

Transformation of normal human kidney cells by retroviral kirsten ras oncogene implications for tumor progression. Proceedings of the American Association for Cancer Research Annual Meeting 29: 445, 1988

Epstein-Barr virus nuclear antigen 2 -oestrogen receptor fusion proteins complement the EBNA2-deficient Epstein-Barr virus strain P3HR1 in transformation of primary B cells but suppress growth of human B cell lymphoma lines. Journal Of General Virology. 77(2): 227-237, 1996

Epstein barr virus anti epstein barr viral capsid antigen immuno globulin m and anti epstein barr nuclear antigen immuno globulin g antibodies titered by immuno fluorescence in micro plates a semi automated method based on micro titer system. Medical Microbiology and Immunology 170(4): 247-254, 1982