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Interrelationship of microsomal and mitochondrial systems for hydroxylating steroids from cattle adrenal cortex: effect of highly-purified components of the mitochondrial monoxygenase system on hydroxylation of steroids by microsomes



Interrelationship of microsomal and mitochondrial systems for hydroxylating steroids from cattle adrenal cortex: effect of highly-purified components of the mitochondrial monoxygenase system on hydroxylation of steroids by microsomes



Biokhimiia 58(11): 1761-1770



The possibility of functioning of microsomal steroid hydroxylases in the adrenal cortex in the presence of the electron-transporting proteins in mitochondrial monooxygenase systems has been studied. It was found that adrenodoxin reductase stimulates both the 21- and 17 alpha-steroid hydroxylation activities in microsomes of the adrenal cortex with the same efficiency as does NADPH-dependent cytochrome P-450 reductase. Using cytochrome b5 and its specific antibody, an attempt has been made to investigate possible mechanisms of the stimulating effect of adrenodoxin reductase.

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Accession: 046443974

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PMID: 8268313


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