+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Long-term administration of L-arginine, L-NAME, and the exogenous NO donor molsidomine modulates urinary nitrate and cGMP excretion in rats

Long-term administration of L-arginine, L-NAME, and the exogenous NO donor molsidomine modulates urinary nitrate and cGMP excretion in rats

Cardiovascular Research 28(4): 494-499

The effects of long term oral administration of L-arginine, NG-nitro-L-arginine methyl-ester (L-NAME), or molsidomine v placebo on blood pressure and the urinary excretion rates of NO3- and cyclic GMP were studied in Munich Wistar Frömter (MWF) rats. L-arginine (2 g.kg-1 body weight, n = 8), NG-nitro-L-arginine methylester (L-NAME; 5 mg.kg-1, n = 8), or molsidomine (3 mg.kg-1, n = 8) were given in drinking water and compared with placebo (n = 8) over a period of five months. Urinary excretion rates of NO3- (by gas chromatography) and cyclic GMP (by radioimmunoassay) were assessed in monthly intervals, as well as systolic blood pressure (tail plethysmography). Mean basal blood pressure was 143.5(SEM 2.2) mm Hg. It was unaffected by L-arginine or molsidomine, but continuously and significantly increased during L-NAME treatment to 199.3(6.4) mm Hg (p < 0.05). Urinary excretion of NO3- increased by 20-41% v controls in L-arginine and molsidomine treated rats (p < 0.05), and decreased by 5-15% in L-NAME treated rats (p < 0.05). Urinary excretion of cyclic GMP increased by 9-38% v controls in the L-arginine and molsidomine treated groups and decreased by 5-20% in the L-NAME treated animals. Consistent with their higher blood pressure, L-NAME treated animals displayed cardiac hypertrophy. Determination of urinary NO3- excretion by gas chromatography is a sensitive and specific method to assess NO formation in vivo. Long term oral administration of L-arginine in MWF rats increases NO production (as assessed by the urinary excretion rates of NO3- and cyclic GMP), but does not significantly influence systolic blood pressure, whereas L-NAME induces sustained hypertension and cardiac hypertrophy due to inhibition of NO formation.

(PDF emailed within 1 workday: $29.90)

Accession: 046567397

Download citation: RISBibTeXText

PMID: 8181036

Related references

Effect of dietary arginine on urinary nitrate excretion in germ-free rats. Food & Chemical Toxicology 34(6): 555-558, 1996

Effects of acute oral loading with L-arginine or L-NAME on urinary excretion rates of nitrate and cyclic GMP in rats. Naunyn-Schmiedeberg's Archives of Pharmacology 349(SUPPL ): R25, 1994

Urinary excretion of arsenic metabolites after long-term oral administration of various arsenic compounds to rats. Journal of Toxicology & Environmental Health Part A 54(3): 179-192, June 12, 1998

Effects on short- and long-term exercise on urinary cGMP excretion in healthy subjects and in patients with coronary artery disease. Journal of Cardiovascular Pharmacology 35(6): 891-896, 2000

Urinary NO3- excretion as an indicator of nitric oxide formation in vivo during oral administration of L-arginine or L-name in rats. Clinical and Experimental Pharmacology and Physiology 23(1): 11-15, 1996

Urinary nitrate excretion in relation to murine macrophage activation. Influence of dietary L-arginine and oral NG-monomethyl-L-arginine. Journal of Immunology 146(4): 1294-1302, 1991

Significance of cadmium excretion in the urine by a long-term cadmium administration to rats. I. Changes in the urinary cadmium level. Sangyo Igaku. Japanese Journal of Industrial Health 19(4): 198-199, 1977

Different effects of short and longer-term arginine vasopressin (AVP) administration on sodium excretion in Brattleboro rats. Acta Physiologica Scandinavica. Supplementum 591: 33-37, 1990

Plasma level and urinary excretion of molsidomine following oral administration of sustained release capsules and conventional tablets to monkeys. Journal of Takeda Research Laboratories 44(1-2): 117-123, 1985

Molsidomine, a nitric oxide donor, modulates rotational behavior and monoamine metabolism in 6-OHDA lesioned rats treated chronically with L-DOPA. Neurochemistry International 63(8): 790-804, 2014

Long-term exercise attenuates blood pressure responsiveness and modulates kidney angiotensin II signalling and urinary sodium excretion in SHR. Journal of the Renin-Angiotensin-Aldosterone System 12(4): 394-403, 2012

Renal excretion of long term sulfonamides under fluid administration and modification of the urinary pH value. Acta Biologica et Medica Germanica 35(6): 793-798, 1976

Marked increase in urinary excretion of nitrate and N-nitrosothioproline in the osteogenic disordered syndrome rats, lacking ascorbic acid biosynthesis, by administration of lipopolysaccharide and thioproline. Carcinogenesis 16(11): 2653-2657, 1995

Short-term black tea intake modulates the excretion of urinary mutagens in rats treated with 2-amino-3-methylimidazo-[4,5-f]quinoline (IQ): role of CYP1A2 upregulation. Archives of Toxicology 78(8): 477-482, 2004

Plasma arginine and urinary nitrate and nitrite excretion in bronchopulmonary dysplasia. Biology of the Neonate 85(3): 173-178, 2003