+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Long-term alcohol self-administration and alcohol withdrawal differentially modulate microtubule-associated protein 2 (MAP2) gene expression in the rat brain

Long-term alcohol self-administration and alcohol withdrawal differentially modulate microtubule-associated protein 2 (MAP2) gene expression in the rat brain

Brain Research. Molecular Brain Research 62(2): 196-205

Chronic alcohol intoxication is known to produce neuronal degeneration in the central and peripheral nervous system of experimental animals and of humans. It is suggested that various components of the cytoskeleton undergo profound changes following chronic alcohol use and misuse. Here we studied the expression of the neuronal cytoskeletal microtubule-associated protein 2 (MAP2) following long-term alcohol consumption and subsequent alcohol withdrawal. Alcohol-preferring AA (Alko Alkohol) rats with a high voluntary alcohol consumption for a period of 16 months were compared with age-matched control rats without prior experience with alcohol. For comparison, in a second experiment, heterogeneous Wistar rats that also had voluntary access to alcohol for 8 months were examined following alcohol consumption and withdrawal. In situ hybridization and subsequent dot blot and Northern blot analysis for further quantification revealed that chronically alcoholized animals exhibit markedly decreased MAP2 mRNA levels in several parts of the extrapyramidal system (mainly in the caudate putamen, the substantia nigra pars compacta and the globus pallidus), the mesolimbic system, in several hypothalamic nuclei and in the nucleus inferior colliculus. Other areas such as the hippocampus, frontoparietal cortex and cerebellum were less affected by chronic alcohol intake, however, in these regions the MAP2 mRNA levels were increased during alcohol withdrawal. These results suggest that long-term alcohol self-administration affects central neurons involved in motor control via the influence on the integrity of the cytoskeleton and may thus induce motor dysfunction.

(PDF emailed within 0-6 h: $19.90)

Accession: 046567439

Download citation: RISBibTeXText

PMID: 9813323

DOI: 10.1016/s0169-328x(98)00253-8

Related references

Morphological brain changes induced by alcohol and aging effects of piracetam during during withdrawal following long term alcohol consumption. Racagni, G And J Mendlewicz (Ed ) International Academy For Biomedical And Drug Research, Vol 2 Treatment Of Age-Related Cognitive Dysfunction: Pharmacological And Clinical Evaluation; Meeting, Monte Carlo, Monaco, October 3-5, 1991 Vi+154p S Karger Ag: Basel, Switzerland; New York, New York, Usa Illus 63-68, 1992

Combination of DAT and DBH gene polymorphisms with a family history of alcohol use disorders increases the risk of withdrawal seizures and delirium tremens during alcohol withdrawal in alcohol-dependent men. Zhurnal Nevrologii i Psikhiatrii Imeni S.S. Korsakova 116(12): 68-80, 2018

Long-term administration of tianeptine in depressed patients after alcohol withdrawal. British Journal of Psychiatry. Supplement 1992(15): 66-71, 1992

Withdrawal symptoms in a long-term model of alcohol self-administration in unselected Wistar rats. Alcoholism Clinical & Experimental Research 22(3 ABSTR SUPPL ): 92A, 1998

The expression of alcohol-induced Fos protein and tyrosine hydroxylase after alcohol withdrawal. Alcoholism Clinical & Experimental Research 24(5 Supplement): 222A, 2000

MCH mRNA expression is differentially modulated by ethanol exposure and withdrawal in lines of mice selected for high and low alcohol withdrawal severity. Alcoholism Clinical & Experimental Research 24(5 Supplement): 120A, 2000

Glucose utilization, hexokinase activity and ATP level in erythrocytes in long-term alcohol intoxication, withdrawal period and insulin administration. Byulleten' Eksperimental'noi Biologii i Meditsiny 117(1): 29-32, 1994

Changes in the alcohol drinking pattern during long-term oral alcohol self-administration in the rat. Naunyn-Schmiedeberg's Archives of Pharmacology 353(4 SUPPL ): R102, 1996

Long-term alcohol self-administration with repeated alcohol deprivation phases: An animal model of alcoholism?. Alcohol & Alcoholism 34(2): 231-243, March-April, 1999

Regulation of microtubule-associated protein 2 (MAP2) mRNA expression during rat brain development. Journal of Molecular Neuroscience 1(3): 189-198, 1989

Role of GABA(B) receptor in alcohol dependence: reducing effect of baclofen on alcohol intake and alcohol motivational properties in rats and amelioration of alcohol withdrawal syndrome and alcohol craving in human alcoholics. Neurotoxicity Research 6(5): 403-414, 2004

Chronic alcohol exposure and withdrawal increase MCH mRNA expression in a mouse line selected for high alcohol withdrawal severity. Alcoholism Clinical & Experimental Research 25(5 Supplement A): 106A, 2001

Chronic alcohol exposure and withdrawal alters prefrontal cortex mRNA expression in mouse lines selected for high alcohol withdrawal severity. Alcoholism Clinical & Experimental Research 26(5 Supplement): 54A, 2002

Phosphorylation states of microtubule-associated protein 2 (MAP2) determine the regulatory role of MAP2 in microtubule dynamics. Biochemistry 36(41): 12574-12582, 1997

Assembly of chick brain MAP2-tubulin microtubule protein. Characterization of the protein and the MAP2-dependent addition of tubulin dimers. Biochemical Journal 277: 231-238, 1991