+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Long-term alloreactive T cell lines and clones express a limited T cell receptor repertoire



Long-term alloreactive T cell lines and clones express a limited T cell receptor repertoire



Transplant Immunology 5(2): 122-128



Alloreactive T cells recognize either determinants of the intact donor MHC molecules displayed on the surface of transplanted-cells or peptide fragments of donor antigens associated with self-MHC molecules by means of their T cell receptors (TCR). To investigate the relationship between the TCR beta chain structure and allorecognition, we established and characterized four long-term T cell lines and seven T cell clones derived following a mixed lymphocyte reaction (MLR) between fully histoincompatible DA (RT1a) and LEW (RT1(1)) rat lymph node cells. These DA anti-LEW T cells were phenotypically CD4+, CD8-, alpha beta TCR+ and produced interferon-gamma but not IL-4, consistent with being Th1 CD4+ T cells. As might be expected, these cells were not significantly cytotoxic and did not display suppressor activity. Analysis of the TCR beta chain gene structure revealed a very restricted repertoire in both long-term lines and clones. The TCRBV6S1 gene was present in 15/21 of the alloreactive T cell mRNA transcripts but only 1/12 of unstimulated DA splenic TCR mRNA transcripts (p = 0.0018). Similarly, the TCRBJ2S1 gene was also used frequently in the alloreactive transcripts (17/21) but in only 2/12 unstimulated splenic transcripts (p = 0.0013). Furthermore, all 15 of the alloreactive TCRBV6S1 transcripts had a distinctive four amino acid N region motif not present in any of the unstimulated TCR transcripts (p = 0.0003). These experiments reveal a distinct homogeneity amongst stable allogeneic T cells in culture. If these results reflect the situation in vivo, the possibility exists that specific immunotherapy may be successful in preventing allograft rejection.

(PDF emailed within 0-6 h: $19.90)

Accession: 046567446

Download citation: RISBibTeXText

PMID: 9269034

DOI: 10.1016/s0966-3274(97)80052-4


Related references

A distinct subset of human CD4+ cells with a limited alloreactive T cell receptor repertoire. Journal of Immunology 143(9): 2783-2789, 1989

The repertoire of killer cell Ig-like receptor and CD94:NKG2A receptors in T cells: clones sharing identical ab TCR rearrangement express highly diverse killer cell Ig-like receptor patterns. Journal of Immunology 166(6): 23-32, 2001

The repertoire of killer cell Ig-like receptor and CD94:NKG2A receptors in T cells: clones sharing identical alpha beta TCR rearrangement express highly diverse killer cell Ig-like receptor patterns. Journal of Immunology 166(6): 3923-3932, 2001

Lymphocytes infiltrating rat cardiac allografts express a limited repertoire of T cell receptor Vb genes. Journal of Immunology 151: 28-38, 1993

T-cell receptor repertoire in tumor-infiltrating lymphocytes. Analysis of melanoma-specific long-term lines. Journal of ImmunoTherapy With Emphasis on Tumor Immunology 16(2): 85-94, 1994

Lymphocytes infiltrating rat cardiac allografts express a limited repertoire of T cell receptor V beta genes. Journal of Immunology 151(10): 5228-5238, 1993

Long-term persistence of limited HTLV-I Tax-specific cytotoxic T cell clones in a patient with adult T cell leukemia/lymphoma after allogeneic stem cell transplantation. Journal of Clinical Immunology 32(6): 1340-1352, 2013

Mechanisms of interleukin 2 production t cell proliferation and t cell functions studies with long term cultured t cell lines and clones. Japanese Archives of Internal Medicine 33(7): 231-246, 1986

Long term culture and characterization of alloreactive human t cell clones from renal needle biopsies. Federation Proceedings 44(6): 1882, 1985

Alloreactive T cell recognition of MHC class I molecules. The T cell receptor interacts with limited regions of the MHC class I long a helices. Journal of Immunology 158: 05-12, 1997

Alloreactive T cell recognition of MHC class I molecules: the T cell receptor interacts with limited regions of the MHC class I long alpha helices. Journal of Immunology 158(6): 2805-2812, 1997

Generation of the t lymphocyte receptor repertoire from germline genes limited diversity of alpha and beta chains in tnp specific t cell clones. Immunobiology 173(2-5): 353, 1986

T lymphocyte cloning from rejected human kidney allograft. Recognition repertoire of alloreactive T cell clones. Journal of Immunology 141(12): 4187-4195, 1988

Analysis of the donor-specific cytotoxic T lymphocyte repertoire in a patient with a long term surviving allograft. Frequency, specificity, and phenotype of donor-reactive T cell receptor (TCR)-ab+ and TCR-cd+ clones. Journal of Immunology 144: 88-94, 1990

Analysis of T-cell receptor beta chain repertoire in an alloreactive T-cell line that causes rat cardiac allograft rejection. Journal of Investigative Medicine 44(1): 157A, 1996