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Malate dehydrogenase isozymes (MDH; EC 1.1.1.37) in long-term callus culture of Cereus peruvianus (Cactaceae) exposed to sugar and temperature stress


, : Malate dehydrogenase isozymes (MDH; EC 1.1.1.37) in long-term callus culture of Cereus peruvianus (Cactaceae) exposed to sugar and temperature stress. Biochemical Genetics 35(5-6): 155-164

Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in long-term callus tissue culture of Cereus peruvianus were studied in starch gel electrophoresis to investigate the control of differential Mdh gene expression under sugar and temperature stress. While two cytosol MDH isozymes showed an unchanged phenotype when the callus tissues were transferred to medium maintained at 22 or 37 degrees C and containing different concentrations of sucrose, glucose, and fructose, the different combinations of five mitochondrial MDH (mtMDH) and two micro-body MDH (mbMDH) showed different MDH isozyme patterns in the callus populations. Differential expression of mtMDH isozymes seems to be modulated at the posttranslational level in callus tissues exposed to different concentrations and types of sugar and to high-temperature and low-temperature stress. An inductor effect on the expression of mbMDH isozymes was observed under stress conditions and in long-term callus tissue, and they may also present different responses.

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Accession: 046605080

PMID: 9332710

DOI: 10.1023/A:1021950008544

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Related references

Jorge, I.C.; Mangolin, C.A.; Machado, M.F.P.S., 1997: Malate dehydrogenase isozymes (MDH; EC 1.1.1.37) in long-term callus cultures of Cereus peruvianus (Cactaceae) exposed to sugar and temperature stress. MDH isoenzymes in 4-year-old callus tissue of C. peruvianus were studied by starch gel electrophoresis to investigate the control of differential Mdh expression under sugar and temperature stress. Two soluble cystolic MDH isoenzymes were observed...

Jorge, I.C.; Mangolin, C.A.; Machado, M.F.P.S., 1997: Malate dehydrogenase isozymes in long-term callus culture of Cereus peruvianus exposed to sugar and temperature stress. Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in long-term callus tissue culture of Cereus peruvianus were studied in starch gel electrophoresis to investigate the control of differential Mdh gene expression under sugar and temperature stress....

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