+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Mapping of transcribed sequences on human chromosome 19



Mapping of transcribed sequences on human chromosome 19



Dna Sequence 6(1): 13-26



30 EST/STS have been mapped on human chromosome 19 using a highly specific hncDNA library as a source of transcribed sequences. In addition more than 50 sites constituting 19 families of closely related sequences containing at least one transcribed member each were mapped across the chromosome. Chromosome-19 specific hncDNA clones were hybridized to chromosome 19 cosmids that were previously assembled into contigs covering about 80% of Chr19. The hybridization results were verified by PCR. Such an approach to EST mapping provides information on possible locations of genes as transcribed units of genome and on location of repeated elements used for the priming the hncDNA synthesis. Mapped hncDNA sequences may serve as good starting points for the systematic sequencing of transcribed genomic regions.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 046622473

Download citation: RISBibTeXText

PMID: 8746456

DOI: 10.3109/10425179509074694


Related references

Isolation and characterization of transcribed sequences from a chromosome 16 hn-cDNA library and the physical mapping of genes and transcribed sequences using a high-resolution somatic cell panel of human chromosome 16. Genomics 20(2): 169-175, 1994

Identification of novel transcribed sequences on human chromosome 22 by expressed sequence tag mapping. Dna Research 8(1): 1-9, 2001

Mapping of genes and transcribed sequences in a gene rich 400-kb region on human chromosome 11p15.1-->p14. Cytogenetics and Cell Genetics 92(1-2): 103-107, 2001

Isolation of new genes mapping to chromosome 12p evaluation of an arrayed hncDNA library with human transcribed 12p sequences. Cytogenetics & Cell Genetics 61(4): 252, 1992

Mapping of genes and transcribed sequences in a gene rich 400-kb region on human chromosome 11p15.1 fwdarw p14. Cytogenetics & Cell Genetics 92(1-2): 103-107, 2001

Mapping of genes and transcribed sequences in a gene rich 400-kb region on human chromosome 11p15.1→p14. Cytogenetic and Genome Research 92(1-2): 103-107, 2001

Rapid isolation and mapping of transcribed sequences on chromosome 21. American Journal of Human Genetics 41(3 Suppl.): A240, 1987

The isolation of transcribed sequences from the human x chromosome. Cytogenetics & Cell Genetics 40(1-4): 721, 1985

Isolation and localization of transcribed sequences on human chromosome 22. Cytogenetics and Cell Genetics 71(1): 81-85, 1995

Identification and isolation of transcribed human X chromosome DNA sequences. Nucleic Acids Research 11(22): 7961-7979, 1983

Identification and characterization of transcribed sequences on human chromosome 9q32-34. Journal of Molecular Neuroscience 5(3): 165-179, 1994

Isolation and characteristics of an ordered library of transcribed sequences of human chromosome 19 from hybrid human-hamster cells. Bioorganicheskaia Khimiia 20(8-9): 919-931, 1994

Identification of human chromosome 22 transcribed sequences with ORF expressed sequence tags. Proceedings of the National Academy of Sciences of the United States of America 97(23): 12690-3, 2000

YAC-assisted cloning of transcribed sequences from the human chromosome 3p21 region. Human Molecular Genetics 2(6): 791-796, 1993

Isolation of genomic clones homologous to transcribed sequences from human X chromosome. Somatic Cell and Molecular Genetics 10(6): 561-571, 1984