+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Matrix-assisted laser desorption/ionization mass spectra reflect solution-phase zinc finger peptide complexation



Matrix-assisted laser desorption/ionization mass spectra reflect solution-phase zinc finger peptide complexation



Journal of the American Society for Mass Spectrometry 10(1): 27-34



The complexation between an 18-residue zinc finger peptide of CCHC type (CCHC = Cys-X2-Cys-X4-His-X4-Cys, X = variable amino acid) from the gag protein p55 of human immunodeficiency virus type 1 (HIV-1) and various transition metal ions was studied by means of circular dichroism spectroscopy and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). A correlation between the complexation behavior in solution and in MALDI-MS could be established. It was shown that MALDI-MS is a fast method suitable for studying metal binding properties of zinc finger complexes.

(PDF emailed within 0-6 h: $19.90)

Accession: 046631507

Download citation: RISBibTeXText

PMID: 9888182

DOI: 10.1016/S1044-0305(98)00116-0


Related references

Detection of specific zinc-finger peptide complexes with matrix-assisted laser desorption/ionization mass spectrometry. Journal of Protein Chemistry 17(6): 517-518, 1998

Detection of Specific Noncovalent Zinc Finger Peptide-Oligodeoxynucleotide Complexes by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. Angewandte Chemie 37(24): 3430-3432, 2018

Quantitative reproducibility of mass spectra in matrix-assisted laser desorption ionization and unraveling of the mechanism for gas-phase peptide ion formation. Journal of Mass Spectrometry 48(3): 299-305, 2013

Evaluation of combined matrix-assisted laser desorption/ionization time-of-flight and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry experiments for peptide mass fingerprinting analysis. Rapid Communications in Mass Spectrometry 25(13): 1881-1892, 2011

Chemically-assisted fragmentation combined with multi-dimensional liquid chromatography and matrix-assisted laser desorption/ionization post source decay, matrix-assisted laser desorption/ionization tandem time-of flight or matrix-assisted laser desorption/ionization tandem mass spectrometry for improved sequencing of tryptic peptides. European Journal of Mass Spectrometry 11(2): 169-179, 2005

Bare silica nanoparticles as concentrating and affinity probes for rapid analysis of aminothiols, lysozyme and peptide mixtures using atmospheric-pressure matrix-assisted laser desorption/ionization ion trap and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry 22(3): 283-290, 2008

Discerning matrix-cluster peaks in matrix-assisted laser desorption/ionization time-of-flight mass spectra of dilute peptide mixtures. Journal of the American Society for Mass Spectrometry 11(1): 88-93, 2000

Precalibration of matrix-assisted laser desorption/ionization-time of flight spectra for peptide mass fingerprinting. Proteomics 2(10): 1365-1373, 2002

Calibration of matrix-assisted laser desorption/ionization time-of-flight peptide mass fingerprinting spectra. Methods in Molecular Biology 367: 49-60, 2006

Importance of matrix:analyte ratio for buffer tolerance using 2,5-dihydroxybenzoic acid as a matrix in matrix-assisted laser desorption/ionization-Fourier transform mass spectrometry and matrix-assisted laser desorption/ionization-time of flight. Journal of the American Society for Mass Spectrometry 9(8): 805-813, 1998

A new method to analyze matrix-assisted laser desorption/ionization time-of-flight peptide profiling mass spectra. Rapid Communications in Mass Spectrometry 19(7): 865-870, 2005

Identification of proteins separated by one-dimensional sodium dodecyl sulfate/polyacrylamide gel electrophoresis with matrix-assisted laser desorption/ionization ion trap mass spectrometry; comparison with matrix-assisted laser desorption/ionization time-of-flight mass fingerprinting. Rapid Communications in Mass Spectrometry 17(17): 1995-2004, 2003

Identification of acetylation and methylation sites of histone H3 from chicken erythrocytes by high-accuracy matrix-assisted laser desorption ionization-time-of-flight, matrix-assisted laser desorption ionization-postsource decay, and nanoelectrospray ionization tandem mass spectrometry. Analytical Biochemistry 306(2): 259-269, July 15, 2002

A convenient method to extract matrix-assisted laser desorption/ionization mass spectrometry spectra from phosphate-containing peptide mixtures. Proteomics 4(7): 1935-1938, 2004

Improving sample treatment for in-solution protein identification by peptide mass fingerprint using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Journal of Proteome Research 6(9): 3393-3399, 2007