+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Membrane signaling and progesterone in female and male osteoblasts. II. Direct involvement of G alpha q/11 coupled to PLC-beta 1 and PLC-beta 3



Membrane signaling and progesterone in female and male osteoblasts. II. Direct involvement of G alpha q/11 coupled to PLC-beta 1 and PLC-beta 3



Journal of Cellular Biochemistry 79(2): 173-181



We have shown that progesterone (10 pM-10 nM) and progesterone covalently bound to bovine serum albumin (P-CMO BSA; 100 pM-1 microM) rapidly increased (within 5 s) the cytosolic free Ca(2+) concentration and inositol 1,4,5 trisphosphate (InsP(3)) formation in confluent female and male rat osteoblasts via a pertussis toxin-insensitive G-protein. The activation of G-proteins coupled to effectors such as phospholipase C (PLC) is an early event in the signal transduction pathway leading to InsP(3) formation. We used antibodies against the various PLC isoforms to show that only PLC-beta1 and PLC-beta 3 were involved in the Ca(2+) mobilization and InsP(3) formation induced by both progestins in female and male osteoblasts, whereas PLC-beta 2, PLC-gamma 1, and PLC-gamma 2 were not. We also used antibodies against the subunits of heterotrimeric G-proteins to show that the activation of PLC-beta 1 and PLC-beta 3 by both progestins involved the G alpha q/11 subunit, which was insensitive to pertussis toxin, whereas G alpha i, G alpha s, and G beta gamma subunits were not. The membrane effects were independent of the concentration of nuclear progesterone receptor, because the concentration of nuclear progesterone receptors was lower in male than in female osteoblasts. These data suggest that progesterone and P-CMO BSA, which does not enter the cell, directly activate G-protein leading to the very rapid formation of second messengers without involving the nuclear receptor.

(PDF emailed within 1 workday: $29.90)

Accession: 046661686

Download citation: RISBibTeXText

PMID: 10967545


Related references

Membrane signalling and progesterone in female and male osteoblasts. I. Involvement of intracellular Ca2+, inositol trisphosphate, and diacylglycerol, but not cAMP. Journal of Cellular Biochemistry 79(2): 334-345, 2 August, 2000

The obligatory intermediacy of 16,17 alpha- and 16,17 beta-epoxides in the biotransformation of androsta-5,16-dien-3 beta-ol to androst-5-ene-3 beta, 16 alpha, 17 beta- and -3 beta, 16 beta, 17 alpha-triols by male rat liver microsomes. Journal of Biological Chemistry 260(15): 8716-8720, 1985

Plasma testosterone in the male, progesterone and estradiol-17 beta in the female, and DELTA 5-3 beta -hydroxysteroid dehydrogenase (3 beta -HSD) activity in the testis and ovary of the Snell dwarf mouse. Biology of Reproduction 23(4): 887-892, 1980

Induction of epinephrine forming enzyme by gluco corticoids steroid hydroxylation and inductive effect cholesterol pregnenolone progesterone 11 alpha hydroxy progesterone 17 alpha hydroxy progesterone 11 beta hydroxy progesterone 21 hydroxy progesterone 11 beta 17 di hydroxy progesterone 17 alpha 21 di hydroxy progesterone sesame d oil metab enz phenylethanolamine n methyl transferase rat. Nature (London) 219(5152): 392-394, 1968

Effect of 5 alpha-androstane-3 beta,17 beta-diol on estradiol-17 beta reception in pituitary cytosol of male and female rats. Biokhimiia 48(9): 1479-1482, 1983

Polarized expression of integrin receptors (alpha 6 beta 4, alpha 2 beta 1, alpha 3 beta 1, and alpha v beta 5) and their relationship with the cytoskeleton and basement membrane matrix in cultured human keratinocytes. Journal of Cell Biology 112(4): 761-773, 1991

Pharmacological characterization of recombinant human neuronal nicotinic acetylcholine receptors h alpha 2 beta 2, h alpha 2 beta 4, h alpha 3 beta 2, h alpha 3 beta 4, h alpha 4 beta 2, h alpha 4 beta 4 and h alpha 7 expressed in Xenopus oocytes. Journal of Pharmacology and Experimental Therapeutics 280(1): 346-356, 1997

Synergistic Ca2+ responses by G{alpha}i- and G{alpha}q-coupled G-protein-coupled receptors require a single PLC{beta} isoform that is sensitive to both G{beta}{gamma} and G{alpha}q. Journal of Biological Chemistry 286(2): 942-951, 2011

Involvement of alpha v beta 5 integrin in the establishment of autocrine TGF-beta signaling in dermal fibroblasts derived from localized scleroderma. Journal of Investigative Dermatology 126(8): 1761-1769, 2006

Nuclear magnetic resonance identification of the taurine conjugate of 3 alpha,6 beta,7 beta-trihydroxy-5 beta,22-cholen-24-oic acid (tauro-delta 22-beta-muricholate) in the serum of female rats treated with alpha-naphthylisothiocyanate. Journal of Lipid Research 34(4): 651-661, 1993