+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Negative Ham's test and sugar water test on admission in paroxysmal nocturnal hemoglobinuria



Negative Ham's test and sugar water test on admission in paroxysmal nocturnal hemoglobinuria



Japanese Journal of Clinical Hematology 35(9): 898-901



A 55-year-old man was admitted to our department because of shortness of breath and pancytopenia on March 27, 1992. On admission, palpebral conjuctiva were anemic. Laboratory findings showed pancytopenia, a reticulocyte count of 44,835/microliters, hemosiderinuria, LDH of 710 IU/l, haptoglobin of 6 mg/dl, NAP score of 301, red cell acetylcholinesterase of 1.5 U, negative Ham's test and negative sugar water test. Bone marrow was hypoplastic (2.4 x 10(4)/microliters). The erythrocytes of this patient showed complement-sensitive cells by the complement lysis sensitivity test, and a negative population consisting of double negative erythrocytes by flow cytometric two-color analysis using monoclonal antibodies to CD55 and CD59. A diagnosis of PNH with hypoplastic bone marrow was thus made. Therefore, flow cytometric two-color analysis appears to be superior to the standard tests currently used.

(PDF emailed within 1 workday: $29.90)

Accession: 046770675

Download citation: RISBibTeXText

PMID: 7967061


Related references

The "sugar-water" test for paroxysmal nocturnal hemoglobinuria. New England Journal of Medicine 275(3): 155-157, 1966

Positivity of the sugar-water test in the screening for paroxysmal nocturnal hemoglobinuria. Blood 84(4): 1349-1349, 1994

Paroxysmal nocturnal hemoglobinuria proved by "sweetened water" test. Vojnosanitetski Pregled 26(12): 638-640, 1969

Paroxysmal nocturnal hemoglobinuria: a case report with a negative Ham presumptive test associated with serum properdin deficiency. Blood 13(11): 1074-1084, 1958

Use of the saccharose test in paroxysmal nocturnal hemoglobinuria. Vnitrni Lekarstvi 23(2): 155-158, 1977

Gel-test or the diagnosis of paroxysmal nocturnal hemoglobinuria. Vox Sanguinis 78(Suppl 1): P084, July, 2000

Use of a gel test for the diagnosis of paroxysmal nocturnal hemoglobinuria. British Journal of Haematology 93(SUPPL 2): 194, 1996

Paroxysmal nocturnal hemoglobinuria with reference to a specific test. Ugeskrift for Laeger 113(50): 1696-1698, 1952

A potential rapid screening test for paroxysmal nocturnal hemoglobinuria. New England Journal of Medicine 288(20): 1059-1060, 1973

Rapid diagnosis of paroxysmal nocturnal hemoglobinuria by gel test agglutination. Revue Francaise de Transfusion et D'hemobiologie 36(2): 135-147, 1993

Ham's test with properdin-deficient serum in nocturnal paroxysmal hemoglobinuria. Polski Tygodnik Lekarski 13(46): 1815-1817, 1958

Evaluation of the sucrose test for diagnosis of paroxysmal nocturnal hemoglobinuria. Problemy Gematologii i Perelivaniia Krovi 17(8): 55-57, 1972

Diagnostic specificity of sucrose hemolysis test for paroxysmal nocturnal hemoglobinuria. Blood 35(4): 462-475, 1970

Paroxysmal nocturnal hemoglobinuria. Diagnosis by acetylcholinesterase and Ham's test in different cell populations. Israel Journal of Medical Sciences 12(1): 76-78, 1976