+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Nitric oxide synthase gene and protein expression are upregulated by Bacille Calmette-Guérin in the rat bladder



Nitric oxide synthase gene and protein expression are upregulated by Bacille Calmette-Guérin in the rat bladder



European Urology 39(3): 349-356



Objective: We hypothesize that Bacille Calmette-Guerin (BCG) may act through the regulation of various isoforms of nitric oxide synthase (NOS) (inducible (iNOS), endothelial (eNOS), and neuronal (nNOS)) genes and protein expression in rat bladder. Methods: Adult female Sprague-Dawley rats (200-250 g) were injected transurethrally with BCG (22 rats) and phosphate-buffered saline (22 control rats), and after 2, 4, 6, and 12 h, and 1, 2, 3, 5, 7, 10, and 14 days, the bladders were harvested. Normal and BCG-treated rat bladders were analyzed for mRNA expression for iNOS, eNOS, and nNOS by reverse-transcriptase polymerase chain reaction. Protein expression was determined by Western blotting and immunohistochemistry. Results: mRNA expression for iNOS was induced after 2 h of BCG injection in the rat bladder. Gene expression for iNOS was highest at 6 h to 1 day followed by decreased expression, reaching its lowest level at 5 days. eNOS mRNA expression was detected in control bladders, but its level was higher in the BCG-treated animals. nNOS mRNA expression was present in all samples, but did not change after BCG treatment. Western blotting confirmed these findings. Immunohistochemistry analysis demonstrated that eNOS was present mainly in endothelium, while iNOS was detected in stroma and nNOS in epithelium and smooth muscle of the rat bladder. Conclusion: The present study demonstrates, for the first time, that BCG treatment up-regulates gene and protein expression of iNOS and eNOS in normal rat bladders, suggesting that BCG action may be mediated through NOS pathways.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 046806215

Download citation: RISBibTeXText

PMID: 11275732


Related references

Nitric Oxide Synthase Gene and Protein Expression Are Upregulated by Bacille Calmette–Guérin in the Rat Bladder. European Urology 39(3): 349-356, 2001

Gemcitabine versus bacille Calmette-Guérin after initial bacille Calmette-Guérin failure in non-muscle-invasive bladder cancer: a multicenter prospective randomized trial. Cancer 116(8): 1893-1900, 2010

Combination of Intravesical Bacille Calmette-Guérin and Chemotherapy vs. Bacille Calmette-Guérin Alone in Non-muscle Invasive Bladder Cancer: A Meta-Analysis. Frontiers in Oncology 9: 121, 2019

Endophthalmitis from Mycobacterium bovis-bacille Calmette-Guerin after intravesicular bacille Calmette-Guerin injections for bladder carcinoma. American Journal of Ophthalmology 128(5): 648-650, 1999

Bacillus Calmette-Guerin induces long-term local formation of nitric oxide in the bladder via the induction of nitric oxide synthase activity in urothelial cells. Journal of Urology 165(2): 678-682, 2001

Lethal Mycobacterium bovis Bacillus Calmette Guerin infection in nitric oxide synthase 2-deficient mice: Cell-mediated immunity requires nitric oxide synthase 2. Laboratory Investigation 80(9): 1385-1397, 2000

Impact of previous bacille Calmette-Guérin failure pattern on subsequent response to bacille Calmette-Guérin plus interferon intravesical therapy. Urology 71(2): 297-301, 2008

Bacille Calmette-Guerin disease following Bacille Calmette-Guerin vaccination of an HIV-infected health-care worker. International Journal of Std and Aids 23(7): E1-E2, 2013

The effect of intravesical Bacillus Calmette-Guerin instillations on the expression of inducible nitric oxide synthase in humans. Nitric Oxide 13(1): 36-41, 2005

Impact of Bacille-Calmette Guerin on the specificity of nuclear matrix protein 22 for recurrent bladder cancer. Journal of Urology 161(4 SUPPL ): 120, 1999

The prognostic significance of p53, p63 and her2 expression in non-muscle-invasive bladder cancer in relation to treatment with bacille Calmette-Guerin. Arab Journal of Urology 13(3): 225-230, 2015

Interferon-gamma inducible protein-10 Quantitative immuno-localisation in bladder cancer before and after intravesical bacille calmette-guerin. European Urology Suppl.s 1(1): 41, 2002

Antitumor activity of recombinant Bacille Calmette-Guérin secreting interleukin-15-Ag85B fusion protein against bladder cancer. International Immunopharmacology 35: 327-331, 2017

Bacillus Calmette-Guerin up-regulates iNOS gene and protein expression in rat bladder. Journal of Urology 161(4 SUPPL ): 118, 1999

Fluid shear stress upregulated endothelial nitric oxide synthase gene expression and nitric oxide formation in human endothelial progenitor cells. Zhonghua Xin Xue Guan Bing Za Zhi 35(4): 359-362, 2007