NoRC--a novel member of mammalian ISWI-containing chromatin remodeling machines

Strohner, R.; Nemeth, A.; Jansa, P.; Hofmann-Rohrer, U.; Santoro, R.; Längst, G.; Grummt, I.

EMBO Journal 20(17): 4892-4900


ISSN/ISBN: 0261-4189
PMID: 11532953
DOI: 10.1093/emboj/20.17.4892
Accession: 046809426

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Transcription by RNA polymerase I on nucleosomal templates requires binding of the transcription termination factor TTF-I to a cognate site 160 bp upstream of the transcription start site. Binding of TTF-I is accompanied by changes in the chromatin architecture which suggests that TTF-I recruits a remodeling activity to the rDNA promoter. We have cloned a cDNA that encodes TIP5 (TTF-I-interacting protein 5), a 205 kDa protein that shares a number of important protein domains with WSTF (Williams syndrome transcription factor) and hAcf1/WCRF180, the largest subunits of human chromatin remodeling complexes hCHRAC and WCRF. TIP5 co-localizes with the basal RNA polymerase I transcription factor UBF in the nucleolus and is associated with SNF2h. The cellular TIP5-SNF2h complex, termed NoRC (nucleolar remodeling complex), induces nucleosome sliding in an ATP- and histone H4 tail-dependent fashion. The results suggest that NoRC is a novel nucleolar chromatin remodeling machine that may serve a role in the regulation of the rDNA locus.