+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Analysis of swine leukocyte antigen class I gene profiles and porcine endogenous retrovirus viremia level in a transgenic porcine herd inbred for xenotransplantation research



Analysis of swine leukocyte antigen class I gene profiles and porcine endogenous retrovirus viremia level in a transgenic porcine herd inbred for xenotransplantation research



Journal of Veterinary Science 19(3): 384-392



Molecular characterization of swine leukocyte antigen (SLA) genes is important for elucidating the immune responses between swine-donor and human-recipient in xenotransplantation. Examination of associations between alleles of SLA class I genes, type of pig genetic modification, porcine endogenous retrovirus (PERV) viral titer, and PERV subtypes may shed light on the nature of xenograft acceptance or rejection and the safety of xenotransplantation. No significant difference in PERV gag RNA level between transgenic and non-transgenic pigs was noted; likewise, the type of applied transgene had no impact on PERV viremia. SLA-1 gene profile type may correspond with PERV level in blood and thereby influence infectiveness. Screening of pigs should provide selection of animals with low PERV expression and exclusion of specimens with PERV-C in the genome due to possible recombination between A and C subtypes, which may lead to autoinfection. Presence of PERV-C integrated in the genome was detected in 31.25% of specimens, but statistically significant increased viremia in specimens with PERV-C was not observed. There is a need for multidirectional molecular characterization (SLA typing, viremia estimation, and PERV subtype screening) of animals intended for xenotransplantation research in the interest of xeno-recipient safety.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 046870098

Download citation: RISBibTeXText

PMID: 29366300


Related references

Porcine endogenous retrovirus transmission characteristics of an inbred herd of miniature swine. Journal of Virology 76(6): 3045-3048, 2002

Human immune responses to porcine endogenous retrovirus-derived peptides presented naturally in the context of porcine and human major histocompatibility complex class I molecules: implications in xenotransplantation of porcine organs. Transplantation 77(10): 1580-1588, 2004

Study of full-length porcine endogenous retrovirus genomes with envelope gene polymorphism in a specific-pathogen-free Large White swine herd. Journal of Virology 74(18): 8575-8581, 2000

Human CD59 incorporation into porcine endogenous retrovirus particles: implications for the use of transgenic pigs for xenotransplantation. Journal of Virology 76(4): 1999-2002, 2002

Absence of interaction between porcine endogenous retrovirus and porcine cytomegalovirus in pig-to-baboon renal xenotransplantation in vivo. Xenotransplantation 25(5): E12395, 2018

No evidence of porcine endogenous retrovirus in patients with type 1 diabetes after long-term porcine islet xenotransplantation. Journal of Medical Virology 82(2): 331-334, 2010

Porcine endogenous retrovirus significantly contributes peptide epitopes for SLA and HLA class I molecules Implications for xenotransplantation. Human Immunology 64(Suppl. 1): S20, 2003

Absence of replication of porcine endogenous retrovirus and porcine lymphotropic herpesvirus type 1 with prolonged pig cell microchimerism after pig-to-baboon xenotransplantation. Journal of Virology 82(24): 12441-8, 2008

Cloning and phylogenetic analysis of envelope gene of porcine endogenous retrovirus derived from miniature swine of China. Chinese Journal of Zoonoses 22(10): 925-928, 2006

Analysis of pig-to-human porcine endogenous retrovirus transmission in a triple-species kidney xenotransplantation model. Transplant International 17(12): 848-858, 2005

Analysis of potential porcine endogenous retrovirus (PERV) transmission in a whole-organ xenotransplantation model without interfering microchimerism. Transplant International 14(1): 31-37, 2001

Heart valves from pigs and the porcine endogenous retrovirus: experimental and clinical data to assess the probability of porcine endogenous retrovirus infection in human subjects. Journal of Thoracic and Cardiovascular Surgery 121(4): 697-701, 2001

Porcine endogenous retrovirus and other viruses in xenotransplantation. Current Opinion in Organ Transplantation 14(2): 175-179, 2009

Detection of porcine endogenous retrovirus in xenotransplantation. Reproductive Biology 14(1): 68-73, 2014

Screening and analysis of porcine endogenous retrovirus in Chinese Banna minipig inbred line. Transplantation Proceedings 36(8): 2485-2487, 2004