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Overexpression of the crgA gene abolishes light requirement for carotenoid biosynthesis in Mucor circinelloides



Overexpression of the crgA gene abolishes light requirement for carotenoid biosynthesis in Mucor circinelloides



European Journal of Biochemistry 267(3): 800-807



This work describes the isolation and characterization of crgA, a Mucor circinelloides gene, which has a dominant-positive effect on light-regulated carotenogenesis. The crgA gene was originally identified in a transformation experiment as a 3'-truncated open reading frame which caused carotenoid overaccumulation in the dark. The complete cloning and sequencing of crgA revealed that its putative product presented several recognizable structural domains: a RING-finger zinc binding domain near the N-terminus, a putative nuclear localization signal, two stretches of acidic amino acids, glutamine-rich regions and a putative isoprenylation motif. The expression of exogenous copies of the complete crgA gene or two different 3'-truncated versions, produced a similar dominant-positive effect on the light-inducible carotenogenesis of M. circinelloides. The presence of these exogenous sequences also caused a missregulation of the endogenous crgA gene, resulting in its overexpression. Collectively, these observations suggest that crgA is involved in the regulation of carotenoid biosynthesis by light.

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Accession: 046897260

Download citation: RISBibTeXText

PMID: 10651817

DOI: 10.1046/j.1432-1327.2000.01058.x


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