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Overexpression, phosphorylation, and growth effects of ORF162, a Klebsiella pneumoniae protein that is encoded by a gene linked to rpoN, the gene encoding sigma 54

Overexpression, phosphorylation, and growth effects of ORF162, a Klebsiella pneumoniae protein that is encoded by a gene linked to rpoN, the gene encoding sigma 54

Fems Microbiology Letters 119(3): 389-394

The product of a Klebsiella pneumoniae gene, orf162, may regulate sigma 54-dependent transcription and has sequence similarity to proteins of the phosphoenolpyruvate-dependent phosphotransferase system (PTS). We have overproduced the product of orf162 and demonstrated its PTS-dependent phosphorylation in Escherichia coli extracts. We have also observed moderate growth inhibition of a wild-type, but not a sigma 54-mutant, strain by overexpression of orf162. These results are consistent with the hypothesis that the product of orf162 could be a regulatory link between the PTS and sigma 54 activity in bacteria.

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Accession: 046897367

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PMID: 8050721

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