+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Overexpression, phosphorylation, and growth effects of ORF162, a Klebsiella pneumoniae protein that is encoded by a gene linked to rpoN, the gene encoding sigma 54



Overexpression, phosphorylation, and growth effects of ORF162, a Klebsiella pneumoniae protein that is encoded by a gene linked to rpoN, the gene encoding sigma 54



Fems Microbiology Letters 119(3): 389-394



The product of a Klebsiella pneumoniae gene, orf162, may regulate sigma 54-dependent transcription and has sequence similarity to proteins of the phosphoenolpyruvate-dependent phosphotransferase system (PTS). We have overproduced the product of orf162 and demonstrated its PTS-dependent phosphorylation in Escherichia coli extracts. We have also observed moderate growth inhibition of a wild-type, but not a sigma 54-mutant, strain by overexpression of orf162. These results are consistent with the hypothesis that the product of orf162 could be a regulatory link between the PTS and sigma 54 activity in bacteria.

(PDF emailed within 1 workday: $29.90)

Accession: 046897367

Download citation: RISBibTeXText

PMID: 8050721


Related references

Cloning and expression in escherichia coli of orf162 a gene from klebsiella pneumoniae encoding a putative negative regulator of sigma 54 dependent transcription. Abstracts of the General Meeting of the American Society for Microbiology 92: 207, 1992

Mutations in genes downstream of the rpoN gene (encoding sigma 54) of Klebsiella pneumoniae affect expression from sigma 54-dependent promoters. Molecular Microbiology 3(12): 1765-1775, 1989

The role of genes downstream of the sigma N structural gene RPON in Klebsiella pneumoniae. Nitrogen fixation: fundamentals and applications Proceedings of the 10th International Congress on Nitrogen Fixation, St Petersburg, Russia, May 28 June 3-1995: 189-194, 1995

Cassette mutagenesis of a proposed dna binding motif in klebsiella pneumoniae rpon sigma 54. Polsinelli, M , R Materassi And M Vincenzini (Ed ) Developments in Plant And Soil Sciences, Vol 48 Nitrogen Fixation; Fifth International Symposium on Nitrogen Fixation With Non-Legumes, Florence, Italy, September 10-14, 1990 Xvii+678p Kluwer Academic Publishers: Dordrecht, Netherlands; Norwell, Massachusetts, Usa Illus 55-56, 1991

Cloning of a gene encoding a unique haemolysin from Klebsiella pneumoniae and its potential use as a species-specific gene probe. Microbial Pathogenesis 33(1): 1-6, July, 2002

Potential transfer of extended spectrum β-lactamase encoding gene, blashv18 gene, between Klebsiella pneumoniae in raw foods. Food Microbiology 60: 39-48, 2016

Detection of ESBLs and AmpC-encoding genes in Escherichia coli, Klebsiella pneumoniae and Klebsiella oxytoca by gene chip technology. Zhongguo Kangshengsu Zazhi 32(12): 727-729,S4, 2007

Mutations in the gene encoding the Sigma 2 subunit of the adaptor protein 1 complex, AP1S2, cause X-linked mental retardation. American Journal of Human Genetics 79(6): 1119-1124, 2006

Regulation of the Caulobacter crescentus rpoN gene and function of the purified sigma 54 in flagellar gene transcription. Molecular & General Genetics 246(6): 697-706, 1995

Cloning, sequencing, and overexpression in Escherichia coli of a sarcosine oxidase-encoding gene linked to the Bacillus creatinase gene. Journal Of Fermentation & Bioengineering. 77(3): 231-234, 1994

Indirect enzyme-linked immunosorbent assay for detection of immunoglobulin G reactive with a recombinant protein expressed from the gene encoding the 116-kilodalton protein of Mycoplasma pneumoniae. Journal of Clinical Microbiology 37(4): 1024-1029, 1999

Effects of overexpression of the alcohol acetyltransferase-encoding gene ATF1 and disruption of the esterase-encoding gene IAH1 on the flavour profiles of Chinese yellow rice wine. International Journal of Food Science & Technology aop(aop), 2012

Mutations in the AP1S2 gene encoding the sigma 2 subunit of the adaptor protein 1 complex are associated with syndromic X-linked mental retardation with hydrocephalus and calcifications in basal ganglia. Journal of Medical Genetics 44(11): 739-744, 2007

Close evolutionary relationship between the chromosomally encoded beta-lactamase gene of Klebsiella pneumoniae and the TEM beta-lactamase gene mediated by R plasmids. Febs Letters 207(1): 69-74, 1986