+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Performance of the APTIMA Combo 2 assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in female urine and endocervical swab specimens



Performance of the APTIMA Combo 2 assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in female urine and endocervical swab specimens



Journal of Clinical Microbiology 41(1): 304-309



The greater sensitivity of nucleic acid amplification tests (NAATs) for Chlamydia trachomatis and Neisseria gonorrhoeae permits the use of urine and other noninvasive specimens, which can increase the reach and decrease the costs of public health screening programs aimed at controlling these infections. This study evaluated the performance of the APTIMA Combo 2 assay, a multiplex assay based on the transcription-mediated amplification reaction, for the simultaneous detection of both pathogens in endocervical swab and urine specimens from females. Combo 2 assay results were compared with patient infected status, which were available by using other commercial NAATs. Sensitivity and specificity for C. trachomatis were 94.2 and 97.6%, respectively, in swabs and 94.7 and 98.9%, respectively, in first-catch urine (FCU). Sensitivity and specificity for N. gonorrhoeae were 99.2 and 98.7%, respectively, in swabs and 91.3 and 99.3%, respectively, in FCU. The assay reliably detected both infections in coinfected patients. The Combo 2 assay can be recommended for use with endocervical swab and urine specimens from females, especially for screening tests for asymptomatic women in sexually transmitted disease surveillance programs. This Food and Drug Administration-cleared assay can be a useful tool in efforts to reduce the prevalence and incidence of C. trachomatis and N. gonorrhoeae infections in sexually active women and to prevent their costly and serious sequelae.

(PDF emailed within 0-6 h: $19.90)

Accession: 046949337

Download citation: RISBibTeXText

PMID: 12517865

DOI: 10.1128/jcm.41.1.304-309.2003


Related references

Comparison of Gen-Probe APTIMA Combo 2 assay to BDProbeTec ET assay for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine and endocervical swab specimens from females. Abstracts of the General Meeting of the American Society for Microbiology 102: 129, 2002

Performance of vaginal swab specimens in the APTIMA Combo 2 assay for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae. Abstracts of the General Meeting of the American Society for Microbiology 102: 129, 2002

Multicenter clinical trial performance of the new Gen-Probe APTIMA Combo 2 assay for detecting Chlamydia trachomatis and Neisseria gonorrhoeae in female and male urine specimens. Clinical Chemistry 47(S6): A97, 2001

Comparison of Gen-Probe APTIMA Combo 2TM assay with Abbott LCx and BDProbeTecTM ET system assays for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in female and male urine specimens. Abstracts of the General Meeting of the American Society for Microbiology 103: C-032, 2003

Clinical evaluation of Gen-Probe APTIMA Combo 2 Assay for C trachomatis and N gonorrhoeae in endocervical swab and urine samples from female patients. Abstracts of the General Meeting of the American Society for Microbiology 103: C-029, 2003

The Effect of Urine Testing in Evaluations of the Sensitivity of the Gen-Probe Aptima??Combo 2 Assay on Endocervical Swabs for Chlamydia trachomatis and Neisseria gonorrhoeae. Sexually Transmitted Diseases 31(5): 273-277, 2004

Initial performance of the new Gen-Probe APTIMATM Combo 2 assay in detecting Chlamydia trachomatis and Neisseria gonorrhoeae from endocervical and urine specimens in women. Abstracts of the General Meeting of the American Society for Microbiology 100: 223, 2000

Comparison of the BD Viper System with XTR Technology to the Gen-Probe APTIMA COMBO 2 Assay using the TIGRIS DTS system for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens. Sexually Transmitted Diseases 39(7): 514-517, 2012

Evaluation of self-collected vaginal swab, first void urine, and endocervical swab specimens for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in adolescent females. Journal of Pediatric and Adolescent Gynecology 21(6): 355-360, 2008

Detection of Neisseria gonorrhoeae and Chlamydia trachomatis in pharyngeal and rectal specimens using the BD Probetec ET system, the Gen-Probe Aptima Combo 2 assay and culture. Sexually Transmitted Infections 85(3): 182-186, 2009

Comparison of the Gen-Probe APTIMA Combo 2 assay to the AMPLICOR CT/NG assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine samples from Australian men and women. Journal of Clinical Microbiology 44(7): 2619-2621, 2006

Comparison of Gen-Probe APTIMA Combo 2 assay for C trachomatis and N gonorrhoeae with LCR, PCR and GC culture on urine swab specimens from men and women. Abstracts of the General Meeting of the American Society for Microbiology 101: 197, 2001

The effect of urine testing in evaluations of the sensitivity of the Gen-Probe Aptima Combo 2 assay on endocervical swabs for Chlamydia trachomatis and neisseria gonorrhoeae: the infected patient standard reduces sensitivity of single site evaluation. Sexually Transmitted Diseases 31(5): 273-277, 2004

Correlation of three commercial nucleic acid amplification techniques and conventional assays for Chlamydia trachomatis and Neisseria gonorrhoeae in female endocervical swab and urine specimens. Abstracts of the General Meeting of the American Society for Microbiology 100: 220, 2000

Comparison of Gen-Probe APTIMA Combo 2 assay for C trachomatis and N gonorrhoeae with ProbeTec and LCx on urine and swab specimens from women using a rotating standard. Abstracts of the General Meeting of the American Society for Microbiology 102: 131, 2002