+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Phenotype and TCR gamma delta variable gene repertoire of intestinal intraepithelial lymphocytes in wild mice (Mus musculus domesticus): abundance of V gamma 1 transcripts and extensive delta gene diversity



Phenotype and TCR gamma delta variable gene repertoire of intestinal intraepithelial lymphocytes in wild mice (Mus musculus domesticus): abundance of V gamma 1 transcripts and extensive delta gene diversity



International Immunology 6(2): 231-238



In order to study murine intestinal intraepithelial lymphocytes (IEL) independent of factors imparted by conditions of laboratory housing and breeding, and to provide a basis for comparison of IEL studies between inbred and outbred mouse populations, IEL from the domestic house mouse, Mus musculus domesticus, were analyzed by flow cytometric analyses using mAbs to murine lymphocyte markers, and by polymerase chain reaction to study the TCR gamma and delta V gene repertoires. The majority of IEL in wild mice were CD3+, CD8+CD4- T cells. CD4+CD8- also were present in IEL isolates from wild mice, although at low numbers. Among IEL, but not T cells from the spleen or lymph nodes, there was a notable lack of Thy-1 expression, a preponderance of CD8 alpha alpha + T cells, and a relatively high ratio (3:1) of TCR gamma delta + T cells over TCR alpha beta + T cells, suggesting that some IEL in wild mice may develop via an extrathymic pathway similar to that described for laboratory mice. Analyses of the IEL gamma and delta variable genes revealed rearrangements of three of six V region gamma genes (V gamma 1, V gamma 2, and V gamma 5), with an abundance of V gamma 1 transcripts as determined by Northern blot analyses. For the delta gene, rearrangement of five of seven V region elements had occurred (V delta 2, V delta 3, V delta 4, V delta 5, and V delta 6).

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 046973618

Download citation: RISBibTeXText

PMID: 8155599


Related references

Phenotype and Tcr- variable gene repertoire of intestinal intraepithelial lymphocytes in wild mice ( Mus musculus domesticus ): abundance of V 1 transcripts and extensive gene diversity. International Immunology 6(2): 231-238, 1994

T cell receptor delta gene repertoire and diversity of intestinal intraepithelial lymphocytes in athymic mice. Molecular Immunology 30(9): 813-819, 1993

Comparison of the t cell receptor variable region gamma gene repertoire of intestinal intra epithelial lymphocytes in inbred and wild mice. FASEB Journal 6(5): A1707, 1992

Interleukin 15 controls the generation of the restricted T cell receptor repertoire of gamma delta intestinal intraepithelial lymphocytes. Nature Immunology 6(12): 1263-1271, 2005

Localization and phenotype of CD3 associated gamma/delta receptor expressing intestinal intraepithelial lymphocytes. Thymus 14(1-3): 31-41, 1989

Gamma delta T lymphocytes in oriental cutaneous leishmaniasis: occurrence and variable delta gene expression. British Journal of Dermatology 128(4): 388-392, 1993

Sequence diversity of gamma delta antigen receptor of intestinal intraepithelial lymphocytes. FASEB Journal 3(3): A484, 1989

A major fraction of human intraepithelial lymphocytes simultaneously expresses the gamma/delta T cell receptor, the CD8 accessory molecule and preferentially uses the V delta 1 gene segment. European Journal of Immunology 21(4): 1053-1059, 1991

Gamma delta tcr positive human intestinal intraepithelial lymphocytes exhibit restricted v gene segment usage and marked oligoclonality. Immunobiology 183(3-4): 280-281, 1991

T cell receptor delta gene rearrangement diversity and expression in murine intestinal intraepithelial lymphocytes iel from athymic mice. FASEB Journal 6(4): A1228, 1992

T-cell receptor gamma delta diversity and specificity of intestinal intraepithelial lymphocytes: analysis of IEL-derived hybridomas. Cellular Immunology 152(2): 305-322, 1993

Coordinated v gamma and v delta gene segment rearrangements in human t cell receptor gamma delta positive lymphocytes. European Journal of Immunology 19(7): 1261-1266, 1989

A novel human v delta gene expressed predominantly in the ti gamma a fraction of gamma delta positive peripheral lymphocytes. European Journal of Immunology 18(12): 2021-2028, 1988

Coordinated V gamma and V delta gene segment rearrangements in human T cell receptor gamma/delta+ lymphocytes. European Journal of Immunology 19(7): 1261-1265, 1989

A novel gamma delta T cell receptor for antigen adds limited diversity to the gamma delta repertoire in adult thymus. Journal of Immunology 142(1): 28-34, 1989