+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Phenotype of Langerhans cells in human afferent skin lymph derived from allergic contact dermatitis



Phenotype of Langerhans cells in human afferent skin lymph derived from allergic contact dermatitis



Experimental Dermatology 2(6): 274-279



By means of microsurgical lymph cannulation human skin lymph derived from the late phase of an elicitation reaction to diphenylcyclopropenone was sampled. Cells were isolated by centrifugation and then treated with mouse anti-CD1a monoclonal antibodies and sheep antimouse antibody-coated Dynabeads. Ultrastructural and immunocytochemical analyses revealed anti-CD1a/Dynabead-rosetted CD1a- and protein S-100-positive cells which did not express monocyte surface markers, but surface antigens such as HLA-DR, ICAM-1 and, in part, LFA-3. In comparison to freshly prepared human epidermal Langerhans cells (LC), a large fraction of these cells contained no or markedly fewer Birbeck granules and exhibited extensive ruffling of the surface. These data suggest that the phenotype of LC in skin lymph derived from the elicitation phase of allergic contact dermatitis is similar to LC cultured in vitro. In the functional concept of LC of our time, these cells correspond to the dendritic cells designated as "veiled".

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 046973671

Download citation: RISBibTeXText

PMID: 8162348

DOI: 10.1111/j.1600-0625.1993.tb00044.x


Related references

Studies on Langerhans cell phenotype in human afferent skin lymph from allergic contact dermatitis. Advances in Experimental Medicine and Biology 378: 523-525, 1995

Different migration patterns of Langerhans cells in skin lymph derived from irritant and allergic contact dermatitis. Journal of Investigative Dermatology 101(3): 422, 1993

Large increase of Langerhans cells in human skin lymph derived from irritant contact dermatitis. British Journal Of Dermatology. 128(2): 184-188, 1993

Rosettes of Langerhans cells and activated T cells in human skin lymph derived from irritant contact dermatitis. Advances in Experimental Medicine and Biology 378: 527-529, 1995

Interleukin-12 expression in human afferent lymph derived from the induction phase of allergic contact dermatitis. British Journal Of Dermatology. 138(2): 297-300,., 1998

Studies on human skin lymph containing Langerhans cells from sodium lauryl sulphate contact dermatitis. Journal of Investigative Dermatology 99(5): 109s-110s, 1992

Analysis of the phenotype and cytokine pattern of blood- and skin-derived nickel specific T cells in allergic contact dermatitis. International Archives of Allergy and Immunology 113(1-3): 384-386, 1997

Human skin lymph derived from irritant and allergic contact dermatitis: interleukin 10 is increased selectively in elicitation reactions. Dermatology 194(3): 221-228, 1997

Ultrastructural image of the Langerhans cells in the skin of patients with allergic contact dermatitis. Przeglad Dermatologiczny 77(4): 241-248, 1990

Activated immunocompetent cells in human skin lymph derived from irritant contact dermatitis: An immunomorphological study. British Journal of Dermatology 132(1): 39-45, 1995

Haptens can directly induce several phenotypic and functional changes of epidermal Langerhans cells required for induction of contact dermatitis in monocyte-derived human Langerhans cells in vitro. Journal of Investigative Dermatology 112(4): 579, 1999

Isolation of human skin-derived lymph: flow and output of cells following sodium lauryl sulphate-induced contact dermatitis. Archives of Dermatological Research 284(3): 123-126, 1992

A comparative study of the expression of cytotoxic proteins in allergic contact dermatitis and psoriasis: spongiotic skin lesions in allergic contact dermatitis are highly infiltrated by T cells expressing perforin and granzyme B. American Journal of Pathology 158(3): 803-808, 2001

IL-1 beta protein in human skin lymph does not discriminate allergic from irritant contact dermatitis. Contact Dermatitis 35(3): 152-156, 1996

Langerhans cells in human allergic contact dermatitis contain varying numbers of birbeck granules. Histochemistry and Cell Biology 94(5): 497-504, 1990