+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Phenotype of in vitro human otosclerotic cells and its modulation by TGF beta

Phenotype of in vitro human otosclerotic cells and its modulation by TGF beta

Cellular and Molecular Biology 41(8): 1039-1049

A study was carried out to obtain a more detailed picture of the phenotypes of human otosclerotic and normal bone cells and to analyse the response of both populations to treatment with TGF beta 1. Total collagen synthesis was found to be decreased, but fibronectin secretion increased in otosclerotic with respect to normal cells. Although overall glycosaminoglycan (GAG) synthesis was lower in otosclerotic cells, the sulphated GAG to hyaluronic acid (HA) ratio was higher, in particular there was greater expression of chondroitin (CS) and dermatan sulphates (DS). TGF beta 1 induced a more marked increase in collagen and fibronectin release and greater production of sulphated GAGs as DS and heparan sulphate (HS) in the otosclerotic cells. The fact that the phenotype of the otosclerotic cells differed from that of the normal cells and could be modified by TGF beta 1 treatment, suggests that TGF beta 1 is implicated in the pathogenesis of otosclerosis.

(PDF emailed within 1 workday: $29.90)

Accession: 046973685

Download citation: RISBibTeXText

PMID: 8747085

Related references

Phenotype characteristics of human B cells studied by Epstein-Barr virus infection. III. In vitro modulation of C3 receptor expression on human lymphoid cells. Immunology Letters 8(6): 349-353, 1984

Phenotype characteristics of human b cells studied by epstein barr virus infection 3 in vitro modulation of complement c 3 receptor expression on human lymphoid cells. 1984

Modulation of phenotype and cytoskeleton architecture by interleukin-1 alpha in human osteoblast-like cells in vitro. Acta Anatomica 145(2): 156-161, 1992

Effects of vitreous on human retinal pigment epithelial rpe cells in vitro modulation of phenotype proliferation and collagen synthesis. Investigative Ophthalmology & Visual Science 32(4): 1056, 1991

TGF-beta differentially regulates extracellular matrix expression in human normal and otosclerotic cells. Acta Anatomica 152(4): 260, 1995

Modulation of cytokeratin expression during in vitro cultivation of human hepatic stellate cells: evidence of transdifferentiation from epithelial to mesenchymal phenotype. Histochemistry and Cell Biology 118(2): 127-136, 2002

Ionizing radiation induces a motile phenotype in human carcinoma cells in vitro through hyperactivation of the TGF-beta signaling pathway. Cellular and Molecular Life Sciences 73(2): 427-443, 2016

Modulation of growth factor incorporation into ECM of human osteoblast-like cells in vitro by 17 beta-estradiol. American Journal of Physiology 267(6 Pt 1): E990-1001, 1994

Differential modulation of basement membrane gene expression in human fibrosarcoma ht 1080 cells by transforming growth factor beta 1 enhanced type iv collagen and fibronectin gene expression correlates with altered culture phenotype of the cells. Laboratory Investigation 64(6): 807-818, 1991

Expression of E-cadherin and beta-catenin in human and rat hepatic stellate cells in vivo and in vitro; evidence of transdifferentiation from epithelial to mesenchymal phenotype. Hepatology 36(4 Part 2): 257A, October, 2002

Modulation of specific beta cell gene (re)expression during in vitro expansion of human pancreatic islet cells. Cell Transplantation 12(7): 799-807, 2003

Modulation of ciliary beat frequency by beta adrenergic and cholinergic agents in human airways epithelial cells in vitro. Clinical & Experimental Allergy 20(SUPPL 1): 92, 1990

Modulation of smooth muscle cell phenotype by endothelial cells and TGF-beta-1. Journal of Cellular Biochemistry Supplement 0(18 PART A): 320, 1994