+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Phenotype of normal cutaneous microvasculature. Immunoelectron microscopic observations with emphasis on the differences between blood vessels and lymphatics

Phenotype of normal cutaneous microvasculature. Immunoelectron microscopic observations with emphasis on the differences between blood vessels and lymphatics

Journal of Investigative Dermatology 106(1): 135-140

The lymphatic system has been poorly characterized in comparison to the blood vessels. We investigated the expression of microvasculature markers in cutaneous lymphatics and blood microvessels in normal skin. Scrotal skin was chosen because of its high density of both types of microvessels. A pre-embedding peroxidase-conjugated immunoelectron microscopy technique was used, allowing both the visualization of the lymph and blood vessels and their immunohistochemical staining. The markers studied included endothelial antigens (recognized by PAL-E, EN-4, and von Willebrand factor/factor VIII-related antigen), structural molecules of the vascular wall (alpha-smooth muscle actin, heparan sulfate proteoglycan, collagen type IV), and adhesion molecules (endothelial leukocyte adhesion molecule-1 [E-selectin], intercellular adhesion molecule-1 [ICAM-1], platelet endothelial adhesion molecule-1 [PECAM-1], vascular cell adhesion molecule-1 [VCAM-1]). It is shown that lymphatics of normal skin are phenotypically different from blood microvasculature, only weakly expressing endothelial markers (EN-4+, von Willebrand factor/factor VIII-related antigen +/-, PAL-E-), mural markers (alpha-smooth muscle actin-, heparan sulfate proteoglycan-, collagen type IV+) and do not express the studied adhesion molecules except PE-CAM-1 (E-selectin-, ICAM-1-, PECAM-1+, VCAM-1-). The results were substantiated by a double-labeling immunoelectron microscopic technique, which facilitates detection and assessment of microvascular segments. By this technique, collagen type IV, recognized by a peroxidase-labeled 2nd antibody, stains the basal lamina by a linear pattern, whereas a second optional epitope is visualized as grains by a silver-enhanced ultra-small gold-conjugated antibody. Our study shows that not only morphology but also antigenic phenotype of lymphatics differs significantly from blood vessels.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 046973693

Download citation: RISBibTeXText

PMID: 8592064

Related references

Localization of fibronectin in the initial lymphatics and blood vessels of normal human skin: an immunoelectron microscopic investigation. Journal of Dermatological Science 4(2): 63-68, 1992

The anchoring of small cutaneous blood vessels electron microscopic observations. Archiv fuer Klinische und Experimentelle Dermatologie 236(3): 217-228, 1970

Innervation of blood vessels in the rat incisor pulp: A scanning electron microscopic and immunoelectron microscopic study. Anatomical Record 251(3): 384-391, 1998

Phenotype of Normal Cutaneous Microvasculature. Journal of Investigative Dermatology 106(1): 135-140, 1996

The role of blood vessels and lymphatics in cutaneous inflammatory processes: an overview. British Journal of Dermatology 109(Suppl. 25): 89-98, 1983

Immunoelectron microscopic localization of HLA-DR antigen on mast cells and vessels in normal and tuberculin-reactive skin. American Journal of Dermatopathology 13(6): 568-574, 1991

Electron microscopic observations on the fine structure of the normal retinal blood vessels in the monkey (Macaca rhesus). Sinai Hospital Journal 10: 72-82, 1961

Electron microscopic observations of pericytes of small blood vessels in the lungs and hearts of normal cattle and swine. Anat Rec 155(4): 513-530, 1966

Kinetic and electron microscopic study on the relationship between collecting lymphatics and surrounding blood vessels in rat mesentery. Lewis, D H (Ed ) Bibliotheca Anatomica, No 18 Current Advances in Basic And Clinical Microcirculatory Research; 10th European Conference on Microcirculation, Cagliari, Italy, Oct 25-29, 1978 X+398p S Karger: Basel, Switzerland, New York, N Y , Usa Illus 31-34, 1979

Kinetic and electron microscopic study on relationship between the collecting lymphatics and the surrounding blood vessels in rat mesentery. Bibliotheca Anatomica 1979(18): 31-34, 1979

The phylogeny of the fine structure of blood vessels and lymphatics: similarities and differences. Lymphology 20(4): 182-188, 1987

Distinguishing lymphatics from blood vessels in normal and diseased skin. Lymphology 20(4): 179-181, 1987

Immunoelectron microscopic observations on the localization of fibronectin in normal human lung. Tohoku Journal of Experimental Medicine 150(2): 209-223, 1986

Immunoelectron microscopic observations on proteoheparan sulfate in normal human lung. Nihon Kyobu Shikkan Gakkai Zasshi 25(7): 738-743, 1987

Blood vessels and nerve fibers in rat incisor pulp. Immunoelectron microscopic observation with anti-substance P antibody. European Journal of Oral Sciences 106(Suppl. 1): 388-391, 1998