+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Phenotypic analyses of limbal epithelial cell cultures derived from donor corneoscleral rims



Phenotypic analyses of limbal epithelial cell cultures derived from donor corneoscleral rims



Clinical and Experimental Ophthalmology 29(3): 138-142



Grafted cultures of limbal epithelial cells aid repair of the corneal epithelium, but their phenotype is unclear. In this study, the phenotype of cultures that were similar in age to those used clinically were analysed. Limbal epithelial cells were isolated from donor corneoscleral rims and grown in various media, including those designed for keratinocytes. Successful cultures in each medium developed predominantly small (10 microm) tightly packed cells. Immunocytochemistry and western blotting revealed expression of keratins 3, 14 and 19. Expression of these keratins in situ was confirmed by immunohistochemistry. Basal limbal epithelial cells were positive for keratins 14 and 19, and suprabasal cells were positive for keratin 3. However intense staining for keratin 14 was also observed at the inner cut edge of corneoscleral rims. These findings demonstrate the potential importance of keratins 14 and 19 as markers of epithelial cell differentiation in the human cornea.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 046973782

Download citation: RISBibTeXText

PMID: 11446453

DOI: 10.1046/j.1442-9071.2001.00394.x


Related references

Epithelial proliferative potential of organ cultured corneoscleral rims; implications for allo-limbal transplantation and eye banking. British Journal of Ophthalmology 90(1): 55-58, 2005

In vivo confocal microscopy assessment of the corneoscleral limbal stem cell niche before and after biopsy for cultivated limbal epithelial transplantation to restore corneal epithelium. Histology and Histopathology 30(2): 183-192, 2015

Phenotypic characterization of human corneal epithelial cells expanded ex vivo from limbal explant and single cell cultures. Experimental Eye Research 79(1): 41-49, 2004

Phenotypic study after cultivated limbal epithelial transplantation for limbal stem cell deficiency. Archives of Ophthalmology 125(10): 1337-1344, 2007

Phenotypic study after cultivated limbal epithelial transplantation for limbal stem cell deficiency. Yearbook of Ophthalmology 2008: 124-125, 2008

Phenotypic alteration of limbal niche-associated limbal epithelial stem cell deficiency by ultraviolet-B exposure-induced phototoxicity in mice. Biochemistry and Cell Biology 91(3): 165-175, 2013

Establishment of Novel Limbus-Derived, Highly Proliferative ABCG2 + /ABCB5 + Limbal Epithelial Stem Cell Cultures. Stem Cells International 2017: 7678637, 2017

Microbial Analysis of Donor Corneoscleral Rims and Storage Media. Ocular Immunology and Inflammation 27(5): 817-820, 2019

Characterisation of human limbal side population cells isolated using an optimised protocol from an immortalised epithelial cell line and primary limbal cultures. Stem Cell Reviews and Reports 10(2): 240-250, 2014

Effect of Cryopreserved Amniotic Membrane Orientation on the Expression of Limbal Mesenchymal and Epithelial Stem Cell Markers in Prolonged Limbal Explant Cultures. Plos one 11(10): E0164408, 2016

Epithelial outgrowth of limbal explant on amniotic membrane exhibits limbal, but not corneal, phenotypic characteristics. ARVO Annual Meeting Abstract Search & Program Planner : Abstract No 2033, 2003

Phenotypic characterization of immortalized normal and primary tumor-derived human prostate epithelial cell cultures. Prostate 44(2): 164-171, 2000

Limbal dermoid epithelium shares phenotypic characteristics common to both hair epidermal and limbal epithelial stem cells. Current Eye Research 38(8): 835-842, 2013

Corneal epithelial cultures generated from organ-cultured limbal tissue: factors influencing epithelial cell growth. Current Eye Research 31(5): 391-399, 2006

Survival of donor epithelial cells after limbal stem cell transplantation. Investigative Ophthalmology and Visual Science 46(3): 803-807, 2005