+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Phenotypic and cytokine analysis of human peripheral blood gamma delta T cells expressing NK cell receptors



Phenotypic and cytokine analysis of human peripheral blood gamma delta T cells expressing NK cell receptors



Journal of Immunology 159(8): 3723-3730



The presence of NK receptors (NKR) on populations of T cells has been proposed to play a regulatory role in T cell function, fine tuning the response to Ag, and influencing the nature of the immune response through rapid secretion of large amounts of cytokines. In this study, we assessed the nature and distribution of NKR on human peripheral blood gamma delta T cells and established clones to study cytokine release. In circulating gamma delta T cells, approximately 80% expressed CD94, approximately 25% expressed NKR-P1A, and approximately 20% expressed p58, values substantially higher than those found on alpha beta T cells from the same donors. When cloned for specific NKR expression, most cells in culture were NKR-P1A+ whereas p58 expression was variable, suggesting that the NKR-P1A phenotype can be acquired in culture whereas expression of p58 is more stable. Some clones were triple positive for CD94, NKR-P1A, and p58. V delta 2+ cells generally expressed a wider range of NKR than V delta 1+ cells. Following activation through CD3, all gamma delta T cell clones released large amounts of IFN-gamma, commencing as early as 4 h postactivation. Some clones also released TNF-alpha and IL-4, but no correlation with specific NKR expression was noted. Activation through NKR-P1A induced moderate levels of IFN-gamma without inducing IL-4. The results suggest that activation of most gamma delta T cells is regulated by signaling events occurring via both the TCR and the NKR. They further show that peripheral blood gamma delta T cells may function as a source of the proinflammatory cytokines IFN-gamma and TNF-alpha.

(PDF emailed within 1 workday: $29.90)

Accession: 046973817

Download citation: RISBibTeXText

PMID: 9378958


Related references

Phenotypic and cytokine analysis of human peripheral blood cd T cells expressing NK cell receptors. Journal of Immunology 159: 23-30, 1997

Isolation and characterization of human peripheral blood CD4+ T cell clones expressing gamma delta T cell receptors. Immunology Letters 29(3): 197-203, 1991

Isolation and characterization of human peripheral blood cd4 positive t cell clones expressing gamma delta t cell receptors. Immunology Letters 29(3): 197-204, 1991

Phenotypic and functional analysis of murine CD3+,CD4-,CD8- TCR-gamma delta-expressing peripheral T cells. Journal of Immunology 142(11): 3754-3762, 1989

Expansion of V-delta-1/V-gamma-8 expressing and V-gamma dual expressing gamma-delta T cells in peripheral blood of patients with inflammatory bowel disease. 9TH INTERNATIONAL CONGRESS OF IMMUNOLOGY [Author] The 9th International Congress of Immunology : 252, 1995

Morphologic and functional characterization of human peripheral blood T cells expressing the T cell receptor gamma/delta. European Journal of Immunology 19(7): 1183-1188, 1989

Segregation of type 1 cytokine production in human peripheral blood lymphocytes: phenotypic differences between IFN-gamma and IL-2-producing cells in the CD8+ T cell subset. European Journal of Immunology 28(11): 3630-3638, 1998

Repertoire analysis of human peripheral blood lymphocytes using a human V delta 3 region-specific monoclonal antibody. Characterization of dual T cell receptor (TCR) delta-chain expressors and alpha beta T cells expressing V delta 3J alpha C alpha-encoded TCR chains. Journal of Immunology 155(6): 3060-3067, 1995

Properties of human T cells expressing the gamma/delta T cell receptors. Paubert-Braquet, M, Dupont, C, Paoletti, R Dynamic Nutrition Research, Vol 1; Foods, nutrition and immunity: Effects of dairy and fermented milk products: 1-12, 1992

Phenotypic analysis of gamma delta t cells in the peripheral blood pb of patients with rheumatoid arthritis ra. Arthritis & Rheumatism 34(9 SUPPL): S176, 1991

Phenotypic analysis of a resting subpopulation of human peripheral blood NK cells: the FcR gamma III (CD16) molecule and NK cell differentiation. Immunology 72(1): 150-157, 1991

Analysis of Fc gamma receptors on human peripheral blood leukocytes by flow microfluorometry. I. Receptor distributions on monocytes, T gamma cells and cells labeled with the 3Al anti-T cell monoclonal antibody. European Journal of Immunology 12(6): 474-479, 1982

Expansion and characterization of human peripheral blood t lymphocytes expressing the gamma delta t cell antigen receptor. FASEB Journal 2(4): ABSTRACT 2168, 1988

Phenotypical heterogeneity among human T cell receptor gamma/delta-expressing clones derived from peripheral blood. European Journal of Immunology 19(11): 2099-2105, 1989

Phenotypic and functional analysis of an elevated subpopulation of gamma delta t cells in the peripheral blood of aids patients pts. Sixth International Conference on Aids Sixth International Conference on Aids, Vols 1-3 Pagination Varies Sixth International Conference on Aids University Of California San Francisco: San Francisco, California, Usa Illus Maps Paper : abstract Sa235, 1990